Regeneration of multilineage skin epithelia by differentiated keratinocytes.
Regeneration of multilineage skin epithelia by differentiated keratinocytes.
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DOI：
10.1038/jid.2009.244
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发表时间：
2010-02
影响因子：
6.5
通讯作者：
Ghazizadeh, Soosan
中科院分区：
文献类型：
--
作者：
Mannik, Jaana;Alzayady, Kamil;Ghazizadeh, Soosan
虽然快速更新的组织如皮肤上皮细胞的稳态是由干细胞维持的，但表皮基底层中干细胞的定向后代保留再生潜力，并且能够响应环境线索形成表皮。然而，尚不清楚表皮谱系角质形成细胞在什么时候失去这种再生潜力。在这项研究中，我们研究了有丝分裂后分化的角质形成细胞的组织形成的程度。我们表明，培养的小鼠角质形成细胞，通过所有措施，分化能够改革自我更新，毛发轴承皮肤移植到合适的网站在体内。结合外皮蛋白驱动的Cre/lox报告系统的遗传标记和谱系追踪研究证实，移植的分化角质形成细胞确实是再生皮肤的来源。更重要的是，对皮肤再生早期阶段的分析显示了移植的分化角质形成细胞的去分化的标志。这些数据表明，致力于分化并不阻止细胞重新进入细胞周期、去分化和获得“干细胞性”。这些发现表明表皮可以使用不同的策略来实现稳态和组织再生。
Although homeostasis of rapidly renewing tissues like skin epithelia is maintained by stem cells, the committed progeny of stem cells in the basal layer of epidermis retain regenerative potential and are capable of forming epidermis in response to environmental cues. It is not clear, however, at what point within the epidermal lineage keratinocytes lose this regenerative potential. In this study, we examined the extent of tissue formation by post-mitotic differentiated keratinocytes. We show that cultures of mouse keratinocytes that were, by all measures, differentiated were able to reform a self-renewing, hair-bearing skin when transplanted onto suitable sites in vivo. Genetic labeling and lineage-tracing studies in combination with an involucrin-driven Cre/lox reporter system confirmed that transplanted differentiated keratinocytes were indeed the source of the regenerated skin. More importantly, analysis of early stages of skin regeneration showed hallmarks of dedifferentiation of transplanted differentiated keratinocytes. These data indicate that commitment to differentiation does not prohibit cells from re-entering the cell cycle, de-differentiating, and acquiring “stemness”. These findings suggest that epidermis can use different strategies for homeostasis and tissue regeneration.
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影响因子：
3.6
作者：
Lu, Z;Ghazizadeh, S
通讯作者：
Ghazizadeh, S
影响因子：
4
作者：
FUCHS, E;BYRNE, C
通讯作者：
BYRNE, C
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5.3
作者：
MILLER, DG;ADAM, MA;MILLER, AD
通讯作者：
MILLER, AD
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作者：
Hadjantonakis, AK;Gertsenstein, M;Nagy, A
通讯作者：
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影响因子：
64.8
作者：
Dor, Y;Brown, J;Melton, DA
通讯作者：
Melton, DA