Transcriptional profiling of the Arabidopsis abscission mutant hae hsl2 by RNA-Seq.
Transcriptional profiling of the Arabidopsis abscission mutant hae hsl2 by RNA-Seq.
复制标题
DOI：
10.1186/1471-2164-14-37
复制
发表时间：
2013-01-17
期刊：
影响因子：
4.4
通讯作者：
Walker JC
中科院分区：
文献类型：
--
作者：
Niederhuth CE;Patharkar OR;Walker JC
脱落是植物响应发育和环境信号而脱落整个器官的机制。拟南芥（Arabidopsis thaliana）是一种仅花器官发育的植物，已被广泛用于研究控制花器官发育的遗传、分子和细胞过程。拟南芥中的脱落需要两个编码功能冗余受体样蛋白激酶的基因，HAESA（HAE）和HAESA-LIKE 2（HSL 2）。双hae hsl 2突变体植物在花发育和成熟的任何阶段都不能使其花器官伸长。使用RNA-Seq，我们比较了野生型和hae hsl 2第15阶段花的转录组，使用富集了增殖区细胞的花托。2034个基因以假发现率调整的p < 0.05差异表达，其中349个具有两倍或更大的表达变化。差异表达的基因富集了水解、细胞壁修饰和防御相关基因。测试脱落中的开花缺乏（ida）突变体中的几个差异表达基因显示，许多相同的基因由IDA和HAE HSL 2共调节，并且支持IDA在HAE和HSL 2信号传导途径中的作用。与来自雄蕊分裂区的微阵列数据的比较显示依赖于HAE HSL 2的基因的表达的不同模式，并揭示HAE HSL 2-独立途径。基因表达的HAE HSL 2依赖性和HAE HSL 2非依赖性变化是消除所需的。HAE和HSL 2影响细胞壁修饰和防御相关基因的表达，这些基因是细胞凋亡所必需的。HAE HSL 2非依赖性基因似乎也在凋亡中起作用，并且另外参与诸如激素信号传导、衰老和胼胝质沉积的过程。
Abscission is a mechanism by which plants shed entire organs in response to both developmental and environmental signals. Arabidopsis thaliana, in which only the floral organs abscise, has been used extensively to study the genetic, molecular and cellular processes controlling abscission. Abscission in Arabidopsis requires two genes that encode functionally redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE 2 (HSL2). Double hae hsl2 mutant plants fail to abscise their floral organs at any stage of floral development and maturation. Using RNA-Seq, we compare the transcriptomes of wild-type and hae hsl2 stage 15 flowers, using the floral receptacle which is enriched for abscission zone cells. 2034 genes were differentially expressed with a False Discovery Rate adjusted p < 0.05, of which 349 had two fold or greater change in expression. Differentially expressed genes were enriched for hydrolytic, cell wall modifying, and defense related genes. Testing several of the differentially expressed genes in INFLORESCENCE DEFICIENT IN ABSCISSION (ida) mutants shows that many of the same genes are co-regulated by IDA and HAE HSL2 and support the role of IDA in the HAE and HSL2 signaling pathway. Comparison to microarray data from stamen abscission zones show distinct patterns of expression of genes that are dependent on HAE HSL2 and reveal HAE HSL2- independent pathways. HAE HSL2-dependent and HAE HSL2-independent changes in genes expression are required for abscission. HAE and HSL2 affect the expression of cell wall modifying and defense related genes necessary for abscission. The HAE HSL2-independent genes also appear to have roles in abscission and additionally are involved in processes such as hormonal signaling, senescence and callose deposition.
登录
查看更多内容
影响因子：
7.4
作者：
González-Carranza, ZH;Whitelaw, CA;Roberts, JA
通讯作者：
Roberts, JA
影响因子：
11.6
作者：
Beisson, Fred;Li, Yonghua;Ohlrogge, John B.
通讯作者：
Ohlrogge, John B.
agriGO：农业界的 GO 分析工具包
影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
Su Z
影响因子：
14.9
作者：
Edgar, R;Domrachev, M;Lash, AE
通讯作者：
Lash, AE
影响因子：
6.9
作者：
Butenko, Melinka A.;Stenvik, Grethe-Elisabeth;Aalen, Reidunn B.
通讯作者：
Aalen, Reidunn B.