Inhibition dominates sensory responses in the awake cortex.
Inhibition dominates sensory responses in the awake cortex.
复制标题
作者：
大脑皮层的活动被认为取决于突触兴奋和抑制之间的精确关系。在视觉皮层，特别是，细胞内的测量有相关的响应选择性，以协调增加兴奋和抑制。然而，这些测量都是在麻醉期间进行的，麻醉强烈影响皮层状态，因此影响感觉处理。清醒时视觉刺激诱发的突触活动尚不清楚。在这里，我们测量了麻醉和清醒小鼠视觉皮层的视觉诱发反应和潜在的突触电导。在麻醉下，反应可以从大范围的视觉空间中引出，并且时间延长。在清醒的反应是更多的空间选择性和更简短。全细胞膜片钳记录的突触电导揭示了一个令人惊讶的差异，在两种条件下的突触抑制。而在麻醉下抑制跟踪兴奋的幅度和空间选择性，在清醒时，它比兴奋强得多，并表现出非常广泛的空间选择性。我们的结论是，在清醒状态下，大脑皮层对视觉刺激的反应主要是由突触抑制，限制其空间传播和时间的持久性。这些结果提供了第一个直接的一瞥突触机制，控制清醒皮层的视觉反应。
The activity of the cerebral cortex is thought to depend on the precise relationship between synaptic excitation and inhibition. In visual cortex, in particular, intracellular measurements have related response selectivity to coordinated increases in excitation and inhibition. These measurements, however, have all been performed during anaesthesia, which strongly influences cortical state and therefore sensory processing. The synaptic activity evoked by visual stimulation during wakefulness is unknown. Here, we measured visually evoked responses – and the underlying synaptic conductances – in the visual cortex of anaesthetised and awake mice. Under anaesthesia, responses could be elicited from a large region of visual space and were prolonged in time. During wakefulness responses were more spatially selective and much briefer. Whole-cell patch-clamp recordings of synaptic conductances revealed a surprising difference in synaptic inhibition during the two conditions. Whereas under anaesthesia inhibition tracked excitation in amplitude and spatial selectivity, during wakefulness it was much stronger than excitation and exhibited extremely broad spatial selectivity. We conclude that during wakefulness cortical responses to visual stimulation are dominated by synaptic inhibition, restricting their spatial spread and temporal persistence. These results provide the first direct glimpse of synaptic mechanisms that control visual responses in the awake cortex.
登录
查看更多内容
影响因子：
16.2
作者：
Atallah BV;Bruns W;Carandini M;Scanziani M
通讯作者：
Scanziani M
特定中间神经元的激活可提高 V1 特征选择性和视觉感知。
影响因子：
64.8
作者：
Lee, Seung-Hee;Kwan, Alex C.;Zhang, Siyu;Phoumthipphavong, Victoria;Flannery, John G.;Masmanidis, Sotiris C.;Taniguchi, Hiroki;Huang, Z. Josh;Zhang, Feng;Boyden, Edward S.;Deisseroth, Karl;Dan, Yang
通讯作者：
Dan, Yang
影响因子：
16.2
作者：
Ozeki, Hirofumi;Finn, Ian M.;Schaffer, Evan S.;Miller, Kenneth D.;Ferster, David
通讯作者：
Ferster, David
影响因子：
25
作者：
Mariño, J;Schummers, J;Sur, M
通讯作者：
Sur, M
影响因子：
16.2
作者：
Crochet, Sylvain;Poulet, James F. A.;Petersen, Carl C. H.
通讯作者：
Petersen, Carl C. H.