Activation of Gossypium hirsutum ACS6 Facilitates Fiber Development by Improving Sucrose Metabolism and Transport.
Activation of Gossypium hirsutum ACS6 Facilitates Fiber Development by Improving Sucrose Metabolism and Transport.
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通过改善蔗糖的代谢和运输，激活山地山脉ACS6的激活可促进纤维的发育。
DOI：
10.3390/plants12203530
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发表时间：
2023-10-11
期刊：
影响因子：
--
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Geng C;Li L;Han S;Jia M;Jiang J
棉纤维产量取决于胚珠表皮上形成的纤维细胞的密度。纤维的起始是由MYB-Mixta样转录因子(GhMMLs)触发的，需要蔗糖供应。乙烯或其前体ACC(1-氨基环丙烷-1-羧酸)可能会影响纤维产量。陆地棉(Gossypium hirsutom(L.)基因组包含35个编码ACC合成酶的ACS基因(GhACS)。在这里，我们探讨了Ghacs家族成员在纤维起始调控中的作用。表达分析表明，GhACS6.3基因对在纤维分化过程中(花前3天到花后5天，−3~5DPA)在胚珠中特异表达，尤其是在−3DPA时表达，而其他GhACS6.3基因的表达水平很低或检测不到。通过qRT-PCR分析，证实了GhACS6.3在纤维发育初期的表达谱。高表达GhACS6.3(GhACS6.3-OE)的转基因株系在胚珠中增加了ACC的积累，从而促进了纤维起始部分和纤维产量构成因素的形成。伴随着GhMML3转录水平的增加，以及编码蔗糖转运体和蔗糖合成酶基因的转录水平增加。这些发现表明，GhACS6.3的激活是纤维最初发育所必需的。本研究结果为进一步研究提高棉纤维产量奠定了基础。
Cotton fiber yield depends on the density of fiber cell initials that form on the ovule epidermis. Fiber initiation is triggered by MYB-MIXTA-like transcription factors (GhMMLs) and requires a sucrose supply. Ethylene or its precursor ACC (1-aminocyclopropane-1-carboxylic acid) is suggested to affect fiber yield. The Gossypium hirsutum (L.) genome contains 35 ACS genes (GhACS) encoding ACC synthases. Here, we explored the role of a GhACS family member in the regulation of fiber initiation. Expression analyses showed that the GhACS6.3 gene pair was specifically expressed in the ovules during fiber initiation (3 days before anthesis to 5 days post anthesis, −3 to 5 DPA), especially at −3 DPA, whereas other GhACS genes were expressed at very low or undetectable levels. The expression profile of GhACS6.3 during fiber initial development was confirmed by qRT-PCR analysis. Transgenic lines overexpressing GhACS6.3 (GhACS6.3-OE) showed increased ACC accumulation in ovules, which promoted the formation of fiber initials and fiber yield components. This was accompanied by increased transcript levels of GhMML3 and increased transcript levels of genes encoding sucrose transporters and sucrose synthase. These findings imply that GhACS6.3 activation is required for fiber initial development. Our results lay the foundation for further research on increasing cotton fiber production.
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DOI：
10.1073/pnas.88.16.7434
发表时间：
1991-08-01
影响因子：
11.1
作者：
HAMILTON, AJ;BOUZAYEN, M;GRIERSON, D
通讯作者：
GRIERSON, D
影响因子：
7.2
作者：
Machado, Adriane;Wu, Yingru;Dennis, Elizabeth S.
通讯作者：
Dennis, Elizabeth S.
DOI：
10.1073/pnas.76.1.170
发表时间：
1979-01-01
影响因子：
11.1
作者：
ADAMS, DO;YANG, SF
通讯作者：
YANG, SF
影响因子：
3
作者：
BEASLEY, CA;TING, IP
通讯作者：
TING, IP
影响因子：
5.4
作者：
Qin, Hongde;Guo, Wangzhen;Zhang, Tianzhen
通讯作者：
Zhang, Tianzhen