Codistribution analysis of elastin and related fibrillar proteins in early vertebrate development.
Codistribution analysis of elastin and related fibrillar proteins in early vertebrate development.
复制标题
早期脊椎动物发育中弹性蛋白和相关纤维蛋白的共分布分析。
DOI：
10.1016/s0945-053x(03)00014-3
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Little,CharlesD
中科院分区：
文献类型：
--
作者：
Visconti,RichardP;Barth,JeremyL;Keeley,FredW;Little,CharlesD
弹性蛋白是一种细胞外基质蛋白，存在于成人和新生儿脉管系统、肺、皮肤和结缔组织中。它以原弹性蛋白的形式分泌，这是一种可溶性蛋白质，在组织间隙中交联形成不溶性弹性蛋白基质。交联弹性蛋白与多种微原纤维相关蛋白（包括 fibrillin-1、fibrillin-2 和 fibulin-1）相关，表明这些蛋白有助于弹性纤维组装、结构或功能。迄今为止，最早报道的弹性蛋白表达是在妊娠 3.5 天发育中的禽类心脏的圆锥干区域。在这里，我们报告弹性蛋白表达在明显较早的发育阶段开始。使用一种新型免疫标记方法，对禽类胚胎发育前 2 天的几个时间点的弹性蛋白、fibrillin-1 和 -2 以及 fibulin-1 的沉积进行了分析。在发育 23 小时时，在与脊索和体节等轴向结构相关的中线发现了弹性蛋白。 Fibrillin-1和-2以及fibulin-1也在这一阶段的胚胎中线表达，其中fibrillin-1和fibulin-1与中线结构周围的纤维中的弹性蛋白高度共定位。通过常规免疫印迹和mRNA检测方法证实了这些基因的表达。我们的结果表明，弹性蛋白多肽沉积发生的时间比之前认识的要早得多。此外，结果表明早期胚胎中线的弹性蛋白沉积伴随着许多细胞外基质多肽的沉积和组织。这些丝状细胞外基质结构可以起到转导或以其他方式稳定胚胎发生过程中产生的动态力的作用。
Elastin is an extracellular matrix protein found in adult and neonatal vasculature, lung, skin and connective tissue. It is secreted as tropoelastin, a soluble protein that is cross-linked in the tissue space to form an insoluble elastin matrix. Cross-linked elastin can be found in association with several microfibril-associated proteins including fibrillin-1, fibrillin-2 and fibulin-1 suggesting that these proteins contribute to elastic fiber assembly, structure or function. To date, the earliest reported elastin expression was in the conotruncal region of the developing avian heart at 3.5 days of gestation. Here we report that elastin expression begins at significantly earlier developmental stages. Using a novel immunolabeling method, the deposition of elastin, fibrillin-1 and -2 and fibulin-1 was analyzed in avian embryos at several time points during the first 2 days of development. Elastin was found at the midline associated with axial structures such as the notochord and somites at 23 h of development. Fibrillin-1 and -2 and fibulin-1 were also expressed at the embryonic midline at this stage with fibrillin-1 and fibulin-1 showing a high degree of colocalization with elastin in fibers surrounding midline structures. The expression of these genes was confirmed by conventional immunoblotting and mRNA detection methods. Our results demonstrate that elastin polypeptide deposition occurs much earlier than was previously appreciated. Furthermore, the results suggest that elastin deposition at the early embryonic midline is accompanied by the deposition and organization of a number of extracellular matrix polypeptides. These filamentous extracellular matrix structures may act to transduce or otherwise stabilize dynamic forces generated during embryogenesis.
登录
查看更多内容
盐皮质激素和阳离子对离体心肌中血管紧张素和去甲肾上腺素正性肌力作用的影响。
影响因子：
4.8
作者：
A. M. Lefer
通讯作者：
A. M. Lefer
狗和猴离体冠状动脉对血管收缩剂反应的区域差异
影响因子：
7.3
作者：
K. Miwa;N. Toda
通讯作者：
N. Toda
血管紧张素 II 的直接心肌作用。
影响因子：
--
作者：
P. Dempsey;Z. T. McCallum;K. Kent;T. Cooper
通讯作者：
T. Cooper
心脏肌膜中血管紧张素 II 受体的鉴定和表征。
DOI：
10.1016/0006-291x(82)91473-5
发表时间：
1982
影响因子：
3.1
作者：
Mukherjee,A;Kulkarni,PV;Haghani,Z;Sutko,JL
通讯作者：
Sutko,JL
影响因子：
8.4
作者：
Karamitopoulou, Eva;Pallante, Pierlorenzo;Fusco, Alfredo
通讯作者：
Fusco, Alfredo