An AGAMOUS MADS-box protein, SlMBP3, regulates the speed of placenta liquefaction and controls seed formation in tomato
An AGAMOUS MADS-box protein, SlMBP3, regulates the speed of placenta liquefaction and controls seed formation in tomato
复制标题
AGAMOUS MADS-box 蛋白 SlMBP3 调节胎盘液化速度并控制番茄种子形成
DOI：
10.1093/jxb/ery418
复制
发表时间：
2018-11
影响因子：
6.9
通讯作者：
Chen Guoping
中科院分区：
文献类型：
--
作者：
Zhang Jianling;Wang Yicong;Naeem Muhammad;Zhu Mingku;Li Jing;Yu Xiaohui;Hu Zongli;Chen Guoping
AGAMOUS (AG) MADS-box转录因子在被子植物的花器官和果实发育中起着重要作用。本研究分离了一个番茄（Solanum lycopersicum） AG MADS-box基因SlMBP3，发现该基因在野生型（WT）、Nr（未成熟）和rin（成熟抑制剂）突变体的花和果实早期发育阶段优先表达。其转录本主要集中在雌蕊中；转录本丰度在种子和胎盘发育期间下降，在花发育期间再次增加。SlMBP3-RNAi番茄植株表现为肉质胎盘，无室凝胶，种子极度畸形，无种皮，而过表达slmbp3的植株表现为胎盘高度液化，种子较大。与WT相比，SlMBP3-RNAi介导的胎盘中细胞壁修饰相关的酶活性、细胞壁成分和色素含量发生了显著变化。这些生理特征在slmbp3 -过表达植物的胎盘中也观察到了变化。SlMBP3-RNAi系成熟种子木质素含量明显低于WT系。RNA-seq和qRT-PCR分析显示，SlMBP3-RNAi系中参与种子发育和细胞壁修饰相关酶的生物合成的基因，即赤霉素、吲哚-3-乙酸和脱落酸，在SlMBP3-RNAi系中下调。综上所述，我们的研究结果表明，SlMBP3参与了番茄胎盘和种子发育的调控。
AGAMOUS (AG) MADS-box transcription factors have been shown to play crucial roles in floral organ and fruit development in angiosperms. Here, we isolated a tomato (Solanum lycopersicum) AG MADS-box gene SlMBP3 and found that it is preferentially expressed in flowers and during early fruit developmental stages in the wild-type (WT), and in the Nr (never ripe) and rin (ripening inhibitor) mutants. Its transcripts are notably accumulated in the pistils; transcripts abundance decrease during seed and placental development, increasing again during flower development. SlMBP3-RNAi tomato plants displayed fleshy placenta without locular gel and extremely malformed seeds with no seed coat, while SlMBP3-overexpressing plants exhibited advanced liquefaction of the placenta and larger seeds. Enzymatic activities related to cell wall modification, and the contents of cell wall components and pigments were dramatically altered in the placentas of SlMBP3-RNAi compared with the WT. Alterations in these physiological features were also observed in the placentas of SlMBP3-overexpressing plants. The lignin content of mature seeds in SlMBP3-RNAi lines was markedly lower than that in the WT. RNA-seq and qRT-PCR analyses revealed that genes involved in seed development and the biosynthesis of enzymes related to cell wall modification, namely gibberellin, indole-3-acetic acid, and abscisic acid were down-regulated in the SlMBP3-RNAi lines. Taking together, our results demonstrate that SlMBP3 is involved in the regulation of placenta and seed development in tomato.
登录
查看更多内容
影响因子：
64.5
作者：
MIZUKAMI, Y;MA, H
通讯作者：
MA, H
DOI：
10.1002/9780470988848.ch9
发表时间：
2007-11
期刊：
--
影响因子：
--
作者：
Shinjiro Yamaguchi;Y. Kamiya;E. Nambara
通讯作者：
Shinjiro Yamaguchi;Y. Kamiya;E. Nambara
影响因子：
4.1
作者：
FRY, SC;SMITH, RC;MATTHEWS, KJ
通讯作者：
MATTHEWS, KJ
影响因子：
1.9
作者：
T. Zabadal;M. J. Bukovac
通讯作者：
T. Zabadal;M. J. Bukovac
影响因子：
6.9
作者：
Marín-Rodríguez, MC;Orchard, J;Seymour, GB
通讯作者：
Seymour, GB