Identification of proteins associated with development of psoriatic arthritis in peripheral blood mononuclear cells: a quantitative iTRAQ-based proteomics study.
Identification of proteins associated with development of psoriatic arthritis in peripheral blood mononuclear cells: a quantitative iTRAQ-based proteomics study.
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外周血单核细胞中与银屑病关节炎发展相关的蛋白质的鉴定：基于 iTRAQ 的定量蛋白质组学研究
DOI：
10.1186/s12967-021-03006-x
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发表时间：
2021-08-03
影响因子：
7.4
通讯作者：
Yan K
中科院分区：
文献类型：
--
作者：
Zhu J;Han L;Liu R;Zhang Z;Huang Q;Fang X;Yang K;Huang G;Zheng Z;Yawalkar N;Deng H;Yan K
用于区分银屑病关节炎（PsA）和银屑病无关节炎（PsO）的生物标志物仍然缺乏。我们应用同量异序标记相对和绝对定量（iTRAQ）和LC-MS/MS分析从PsO患者、PsA患者和健康对照者收集的外周血单个核细胞（PBMC）的蛋白质组谱。通过生物信息学分析和蛋白质印迹分析鉴定差异表达蛋白。在所有银屑病患者与健康对照、PsO组与健康对照、PsA组与健康对照以及PsA组与PsO组的比较中，我们分别鉴定了389、199、291和60个显著差异表达的蛋白质（adj.p < 0.05）。在这些蛋白中，有14个蛋白可能代表PsA的有希望的生物标志物：SIRT 2、NAA 50、ARF 6、ADPRHL 2、SF 3B 6、SH 3 KBP 1、UBA 3、SCP 2、RPS 5、NUDT 5、NCBP 1、SYNE 1、NDUFB 7、HTATSF 1。此外，蛋白质印迹法证实，SIRT 2在PsA患者PBMC中的表达显著高于PsO和健康对照组，并且与p38丝裂原活化蛋白激酶（p-p38 MAPK; p = 0.006，r =-0.582）的磷酸化呈负相关。这项初步研究提供了一个广泛的表征PsA的PBMC的蛋白质组相比，PsO和健康对照，这可能有助于提供前瞻性的策略PsA的诊断。在线版本包含补充材料，可通过10.1186/s12967-021-03006-x获得。
Biomarkers for distinguishing psoriatic arthritis (PsA) from psoriasis without arthritis (PsO) are still lacking. We applied isobaric tags for relative and absolute quantification (iTRAQ) and LC–MS/MS to analyze the proteome profile of peripheral blood mononuclear cells (PBMCs) collected from patients with PsO, patients with PsA, and healthy controls. Bioinformatics analysis and western blotting were performed to identify and validate differentially expressed proteins. We identified 389, 199, 291, and 60 significantly differentially expressed proteins (adj.p < 0.05) in the comparison of all psoriatic patients versus healthy controls, PsO group versus healthy controls, PsA group versus healthy controls, and PsA group versus PsO group, respectively. Among these proteins, 14 proteins may represent promising biomarkers for PsA: SIRT2, NAA50, ARF6, ADPRHL2, SF3B6, SH3KBP1, UBA3, SCP2, RPS5, NUDT5, NCBP1, SYNE1, NDUFB7, HTATSF1. Furthermore, western blotting confirmed that SIRT2 expression was significantly higher in PBMCs from PsA patients than PsO and healthy controls, and was negatively correlated with the phosphorylation of p38 mitogen-activated protein kinase (p-p38MAPK; p = 0.006, r = − 0.582). This pilot study provided a broad characterization of the proteome of PBMCs in PsA as compared to PsO and healthy controls, which may help to provide prospective strategies for PsA diagnosis. The online version contains supplementary material available at 10.1186/s12967-021-03006-x.
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影响因子：
5.8
作者：
Chen, Ming;Xu, Min;Zhou, Feng
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