Effects of miRNA-140 on the Growth and Clinical Prognosis of SMMC-7721 Hepatocellular Carcinoma Cell Line.
Effects of miRNA-140 on the Growth and Clinical Prognosis of SMMC-7721 Hepatocellular Carcinoma Cell Line.
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miRNA-140 对 SMMC-7721 肝癌细胞系生长和临床预后的影响。
DOI：
10.1155/2021/6638915
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发表时间：
2021
影响因子：
--
通讯作者：
Fan XH
中科院分区：
文献类型：
--
作者：
Kong CQ;Chen XC;Qiu GH;Liang JC;Wang D;Liu XY;Liu JJ;Han YQ;Fan XH
越来越多的研究表明，microRNA在多种肿瘤的发生和发展中发挥着重要作用，并在各种生物学过程中起着重要的调节作用。然而，miRNA-140在肝细胞癌（HCC）细胞中的表达和功能尚未得到充分鉴定和证实。 通过实时定量聚合酶链反应（qRT-PCR）测定HCC组织和邻近非肿瘤组织中miRNA-140的表达。采用Kaplan-Meier生存分析和考克斯回归分析探讨miRNA-140表达水平与HCC患者生存率的相关性。此外，进行过表达实验以研究miRNA-140在HCC细胞中的生物学作用。利用生物信息学方法预测miRNA-140的相关靶基因和作用途径。 QRT-PCR结果显示，肝癌组织中miRNA-140的表达水平低于癌旁正常组织（P < 0.0001）。与对照组相比，miRNA-140模拟物组SMMC-7721肝癌细胞的增殖、迁移和侵袭能力降低（P < 0.05），而miRNA-140抑制剂组SMMC-7721肝癌细胞的增殖、迁移和侵袭能力增加（P < 0.05）。细胞周期停滞于G 0/1期。预后分析显示，miRNA-140的表达水平与HCC的预后无关。此外，Kaplan-Meier检验显示，肝癌组织中miRNA-140表达水平较低的患者在肝切除术后的无病生存期（DFS，P = 0.004）和总生存期（OS）时间（P = 0.010）显著较短。考克斯回归分析进一步表明miRNA-140是影响肝切除术后患者DFS（P = 0.004）和OS时间（P = 0.014）的独立危险因素。我们的研究结果表明，miRNA-140可能是参与HCC进展的关键调节因子，因此被认为是HCC的潜在预后生物标志物和治疗靶点。
A growing number of studies have suggested that microRNAs exert an essential role in the development and occurrence of multiple tumours and act as crucial regulators in various biological processes. However, the expression and function of miRNA-140 in hepatocellular carcinoma (HCC) cells are not yet adequately identified and manifested. The expression of miRNA-140 was determined in HCC tissues and adjacent nontumour tissues by quantitative real-time polymerase chain reaction (qRT-PCR). Kaplan–Meier survival analysis and Cox regression analysis were performed to explore the correlation between miRNA-140 expression level and the survival rate of patients with HCC. Additionally, overexpression experiments were conducted to investigate the biological role of miRNA-140 in HCC cells. Bioinformatics was used to predict the related target genes and pathways of miRNA-140. QRT-PCR results signified that the expression level of miRNA-140 in HCC was lower than that of adjacent normal tissues (P < 0.0001). Compared with the control group, the SMMC-7721 HCC cells in the miRNA-140 mimic group had a decrease in proliferation, migration, and invasion (P < 0.05), whereas those in the miRNA-140 inhibitor group had an increase in proliferation, migration, and invasion (P < 0.05). Cell cycle arrest occurred in the G0/1 phase. Prognosis analysis showed that the expression level of miRNA-140 was not related to the prognosis of HCC. Furthermore, the Kaplan–Meier test revealed that patients with lower miRNA-140 expression levels in liver cancer tissue had significantly shorter disease-free survival (DFS, P = 0.004) and overall survival (OS) times (P = 0.010) after hepatectomy. Cox regression analysis further indicated that miRNA-140 was an independent risk factor that may affect the DFS (P = 0.004) and OS times (P = 0.014) of patients after hepatectomy. Our results suggested that miRNA-140 might be a crucial regulator involved in the HCC progression and is thus considered a potential prognostic biomarker and therapeutic target for HCC.
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DOI：
10.1038/nrgastro.2013.143
发表时间：
2014-01
期刊：
Nature reviews. Gastroenterology & hepatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.6
作者：
Cai, Hongqiao;Yang, Yang;Ji, Bai
通讯作者：
Ji, Bai
影响因子：
10.3
作者：
Polley, Eric;Kunkel, Mark;Teicher, Beverly A.
通讯作者：
Teicher, Beverly A.
影响因子：
--
作者：
Su Y;Xiong J;Hu J;Wei X;Zhang X;Rao L
通讯作者：
Rao L
影响因子：
5.8
作者：
Reddy KB
通讯作者：
Reddy KB