In vitromodel to study confined osteocyte networks exposed to flow-induced mechanical stimuli.
In vitromodel to study confined osteocyte networks exposed to flow-induced mechanical stimuli.
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DOI：
10.1088/1748-605x/aca37c
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发表时间：
2022-11-25
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骨细胞被认为是骨组织中的主要机械传感器，并协调相邻的成骨细胞和破骨细胞的耦合骨重塑活动。通过相互连接的骨细胞网络进行机械诱导信号传播的体内研究，由于它们被限制在矿化骨基质中，这在传统的培养系统中无法模拟。在这项研究中，我们开发了一种新的模型，使用甲基丙烯酸明胶（GelMA）水凝胶或使用成骨细胞样模型细胞矿化的GelMA来模拟这种体内约束。该模型还可以实时光学检查骨细胞钙（Ca2+）信号动力学响应流体剪切刺激培养在受限条件下。使用该系统，我们发现了几种不同的和以前描述的Ca2+反应模式，这些模式在相互连接的骨细胞网络中作为空间、时间和连通性的函数而变化。Ca2+信号的异质性可能为响应机械负荷的骨重塑提供新的见解。总的来说，这样的模型可以扩展到在受限条件下暴露于血流诱导的机械刺激的细胞网络内的信号动力学研究。
Osteocytes are considered the primary mechanical sensor in bone tissue and orchestrate the coupled bone remodeling activity of adjacent osteoblast and osteoclast cells. In vivo investigation of mechanically induced signal propagation through networks of interconnected osteocytes is confounded by their confinement within the mineralized bone matrix, which cannot be modeled in conventional culture systems. In this study, we developed a new model that mimics this in vivo confinement using gelatin methacrylate (GelMA) hydrogel or GelMA mineralized using osteoblast-like model cells. This model also enables real-time optical examination of osteocyte calcium (Ca2+) signaling dynamics in response to fluid shear stimuli cultured under confined conditions. Using this system, we discovered several distinct and previously undescribed patterns of Ca2+ responses that vary across networks of interconnected osteocytes as a function of space, time and connectivity. Heterogeneity in Ca2+ signaling may provide new insights into bone remodeling in response to mechanical loading. Overall, such a model can be extended to study signaling dynamics within cell networks exposed to flow-induced mechanical stimuli under confined conditions.
皮质骨弥漫性基质损伤部位溶质转运升高：对骨修复的影响。
DOI：
10.1002/jor.23742
发表时间：
2018-03
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
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作者：
Wang B;Sun X;Akkus O;Wang L
通讯作者：
Wang L