Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis.
Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis.
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DOI：
10.1007/s11103-010-9645-0
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发表时间：
2010-08
影响因子：
5.1
通讯作者：
Cheong, Hyeonsook
中科院分区：
文献类型：
--
作者：
Hwang, Indeok;Kim, Soo Young;Kim, Cheol Soo;Park, Yoonkyung;Tripathi, Giri Raj;Kim, Seong-Ki;Cheong, Hyeonsook
在拟南芥中，茎的分枝和生长受植物激素如生长素等成分的控制。我们发现了一个突变体（igi1），显示出高度下降和束状分支模式。在RNA干扰IGI1基因后，表型恢复为野生型。GUS法组织化学分析显示，IGI1基因在花药中有组织特异性表达，且IGI1基因在包括花在内的根尖部位的表达水平高于植株其他部位。IAA处理下，igi1突变体中生长素生物合成成分基因CYP79B2表达上调，igi1基因表达下调。这些结果表明IGI1与植物激素生长素之间存在相互调节作用。此外，与生长素相关的茎枝分枝调控基因MAX3和MAX4在igi1突变体中表达下调。综上所述，这些结果表明IGI1的过表达影响了MAX途径在茎部分支调节中的作用。本文的在线版本（doi:10.1007/s11103-010-9645-0）包含补充材料，仅供授权用户使用。
Shoot branching and growth are controlled by phytohormones such as auxin and other components in Arabidopsis. We identified a mutant (igi1) showing decreased height and bunchy branching patterns. The phenotypes reverted to the wild type in response to RNA interference with the IGI1 gene. Histochemical analysis by GUS assay revealed tissue-specific gene expression in the anther and showed that the expression levels of the IGI1 gene in apical parts, including flowers, were higher than in other parts of the plants. The auxin biosynthesis component gene, CYP79B2, was up-regulated in igi1 mutants and the IGI1 gene was down-regulated by IAA treatment. These results indicated that there is an interplay regulation between IGI1 and phytohormone auxin. Moreover, the expression of the auxin-related shoot branching regulation genes, MAX3 and MAX4, was down-regulated in igi1 mutants. Taken together, these results indicate that the overexpression of the IGI1 influenced MAX pathway in the shoot branching regulation. The online version of this article (doi:10.1007/s11103-010-9645-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1074/jbc.m805453200
发表时间：
2009-02-20
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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