Intrinsic properties of and thalamocortical inputs onto identified corticothalamic-VPM neurons.
Intrinsic properties of and thalamocortical inputs onto identified corticothalamic-VPM neurons.
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DOI：
10.3109/08990220.2013.869495
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发表时间：
2014-06
影响因子：
0.9
通讯作者：
Brumberg JC
中科院分区：
文献类型：
--
作者：
Yang Q;Chen CC;Ramos RL;Katz E;Keller A;Brumberg JC
皮质丘脑（CT）反馈在调节皮质接收的感觉信息中起着重要作用。在体感觉皮层的第六层中，反馈信号传递给丘脑腹侧后内侧核，后者又接收来自对侧触须的感觉信息。我们研究了大鼠体感觉皮层CT神经元的生理和形态，重点研究了它们从丘脑接收的单突触输入的生理特征。为了鉴定CT神经元，将罗丹明微球注射到VPM中，并允许其逆行转运到CT神经元的胞体。注射后至少3天制备丘脑皮层切片。第六层标记CT细胞的全细胞记录显示它们是规则的尖峰神经元，表现出很少的尖峰频率适应。根据顶端树突的五层（致密细胞）和四层（精细细胞），确定了两种解剖类型。丘脑输入到已识别的CT-VPM神经元上，在宽频率范围内（2-20 Hz）显示成对脉冲抑制。刺激训练在10hz以上也导致显著的突触抑制。我们的研究结果表明，丘脑输入对第六层CT-VPM神经元的影响是不同的。这一特征可能使它们能够区分大范围的刺激频率，从而进一步调整反馈到丘脑的信号。
Corticothalamic (CT) feedback plays an important role in regulating the sensory information that the cortex receives. Within the somatosensory cortex layer VI originates the feedback to the ventral posterior medial (VPM) nucleus of the thalamus, which in turn receives sensory information from the contralateral whiskers. We examined the physiology and morphology of CT neurons in rat somatosensory cortex, focusing on the physiological characteristics of the monosynaptic inputs that they receive from the thalamus. To identify CT neurons, rhodamine microspheres were injected into VPM and allowed to retrogradely transport to the soma of CT neurons. Thalamocortical slices were prepared at least 3 days post injection. Whole-cell recordings from labeled CT cells in layer VI demonstrated that they are regular spiking neurons and exhibit little spike frequency adaption. Two anatomical classes were identified based on their apical dendrites that either terminated by layer V (compact cells) or layer IV (elaborate cells). Thalamic inputs onto identified CT-VPM neurons demonstrated paired pulse depression over a wide frequency range (2–20 Hz). Stimulus trains also resulted in significant synaptic depression above 10 Hz. Our results suggest that thalamic inputs differentially impact CT-VPM neurons in layer VI. This characteristic may allow them to differentiate a wide range of stimulation frequencies which in turn further tune the feedback signals to the thalamus.
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影响因子：
2.5
作者：
Hattox, Alexis M.;Nelson, Sacha B.
通讯作者：
Nelson, Sacha B.
影响因子：
5.3
作者：
Heiss, Jaime E.;Katz, Yonatan;Lampl, Ilan
通讯作者：
Lampl, Ilan
影响因子：
64.8
作者：
CRAIR, MC;MALENKA, RC
通讯作者：
MALENKA, RC
DOI：
10.1073/pnas.91.22.10380
发表时间：
1994-10-25
影响因子：
11.1
作者：
ALLEN, C;STEVENS, CF
通讯作者：
STEVENS, CF
DOI：
10.1126/science.1236425
发表时间：
2013-06-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Constantinople CM;Bruno RM
通讯作者：
Bruno RM