Immunocytochemical localization of protein kinase C isozymes in rat brain
Immunocytochemical localization of protein kinase C isozymes in rat brain
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大鼠脑中蛋白激酶 C 同工酶的免疫细胞化学定位
作者：
Freesia L. Huang;Y. Yoshida;H. Nakabayashi;W. Young;Kuo
最近，我们从大鼠脑中分离到3种蛋白激酶C(PKC)同工酶(Huang et al.，1986a)。使用免疫印迹同工酶特异性抗体，我们已经确定了大鼠大脑不同区域中每种同工酶的相对水平(Huang等人，1987b)。本文用光镜免疫细胞化学方法研究了PKC同工酶在大鼠脑内的细胞分布。PKC抗体染色显示神经元胞体及其树突呈强阳性反应，轴突和星形胶质结构呈弱阳性或无阳性反应。在小脑，I型PKC抗体染色蒲肯野细胞体和树突，II型PKC抗体染色颗粒细胞，III型PKC抗体染色浦肯野细胞和颗粒细胞。在大脑皮层，所有抗体均在第II~VI层染色类似锥体细胞的神经元及其顶端树突，而第I层几乎不染色。然而，各种同工酶特异性抗体显示出不同的层状分布的阳性神经元，并且III型PKC阳性神经元的密度高于I型或II型PKC阳性神经元，尤其是在扣带回(脾后)和梨状皮质的II层。在海马区，海马区锥体细胞和齿状回颗粒细胞均被PKC抗体染色。在亚细胞内，III型PKC主要出现在这些神经元的胞浆中，而I型和II型PKC似乎也与细胞核联系。在嗅球内，II型和III型PKC抗体均染色于球周细胞和颗粒细胞，后者也染色于二尖瓣细胞。PKC同工酶在细胞和亚细胞中的不同分布表明，每种同工酶在不同的神经功能中发挥着独特的作用。
Recently, we isolated 3 protein kinase C (PKC) isozymes from rat brain (Huang et al., 1986a). Using isozyme-specific antibodies for immunoblot, we have determined the relative levels of each isozyme in various regions of the rat brain (Huang et al., 1987b). The present paper describes the cellular distributions of PKC isozymes in rat brain as determined by light microscopic immunocytochemistry. Staining with PKC antibodies revealed strong immunoreactivities in neuronal somata and their dendrites and weak to no reaction in axon and the astroglial structures. In the cerebellum, the type I PKC antibodies stained the Purkinje cell bodies and dendrites; the type II PKC antibodies stained the granule cells; and the type III PKC antibody stained both Purkinje and granule cells. In the cerebral cortex, all antibodies stained neurons resembling pyramidal cells and their apical dendrites in layers II to VI, while layer I was nearly devoid of staining. However, the various isozyme-specific antibodies revealed distinct laminar distribution patterns of the positively stained neurons, and the type III PKC-positive neurons exhibited a higher density than those of type I or II PKC-positive ones, especially in layer II of cingulate (retrosplenial) and piriform cortices. In the hippocampal formation, both pyramidal cells of the hippocampus and granule cells of the dentate gyrus were stained by all PKC antibodies. Subcellularly, type III PKC appeared mostly in the cytoplasm of these neurons, whereas type I and II PKC seemed to associate with the nucleus as well. In the olfactory bulb, both type II and III PKC antibodies stained the periglomerular and granular cells, and the latter also stained the mitral cells. The distinct cellular and subcellular distribution of PKC isozymes suggests that each isozyme plays a unique role in the various neural functions.
蛋白激酶 C 的结构域结构和磷酸化。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mochly-Rosen,D;KoshlandJr,DE
通讯作者：
KoshlandJr,DE
磷脂/Ca2 依赖性蛋白激酶的多克隆抗体以及该酶在大鼠脑中的免疫细胞化学定位。
DOI：
10.1073/pnas.82.9.3030
发表时间：
1985
影响因子：
11.1
作者：
Girard,PR;Mazzei,GJ;Wood,JG;Kuo,JF
通讯作者：
Kuo,JF
蛋白激酶 C 激活导致蛋白 F1 磷酸化，可能通过突触前末端生长调节突触可塑性。
DOI：
10.1016/s0163-1047(85)90184-0
发表时间：
1985
期刊：
Behavioral and neural biology
影响因子：
--
作者：
Routtenberg,A
通讯作者：
Routtenberg,A