Collective chemotaxis requires contact-dependent cell polarity.
Collective chemotaxis requires contact-dependent cell polarity.
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DOI：
10.1016/j.devcel.2010.06.012
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发表时间：
2010-07-20
影响因子：
11.8
通讯作者：
Mayor, Roberto
中科院分区：
文献类型：
--
作者：
Theveneau, Eric;Marchant, Lorena;Kuriyama, Sei;Gull, Mazhar;Moepps, Barbara;Parsons, Maddy;Mayor, Roberto
定向集体迁移现在是胚胎发生和癌症期间广泛认可的迁移模式。然而，一群细胞如何对化学引诱物做出反应还没有完全了解。神经嵴细胞是胚胎中最活跃的细胞之一，它们的行为被比作恶性入侵。在这里，我们表明，神经嵴细胞被集体吸引向趋化因子Sdf 1。虽然不参与初始极化细胞，Sdf 1定向稳定细胞接触促进细胞突起。在这种细胞接触中，N-钙粘蛋白抑制突起和Rac 1活性，反过来促进突起和Rac 1在自由边缘的激活。这些结果显示了N-钙粘蛋白在运动的接触抑制过程中的作用，它们揭示了一种可能在胚胎发生和癌症转移过程中起作用的化学吸引机制，从而吸引物如Sdf 1放大和稳定接触依赖性细胞极性，导致定向集体迁移。神经嵴（NC）细胞对Sdf 1进行集体趋化，NC细胞对Sdf 1的趋化需要N-cadherin依赖性接触，N-cadherin通过控制小GTP酶调节运动的接触抑制，Sdf 1增强N-cadherin/接触抑制诱导的细胞极性
Directional collective migration is now a widely recognized mode of migration during embryogenesis and cancer. However, how a cluster of cells responds to chemoattractants is not fully understood. Neural crest cells are among the most motile cells in the embryo, and their behavior has been likened to malignant invasion. Here, we show that neural crest cells are collectively attracted toward the chemokine Sdf1. While not involved in initially polarizing cells, Sdf1 directionally stabilizes cell protrusions promoted by cell contact. At this cell contact, N-cadherin inhibits protrusion and Rac1 activity and in turn promotes protrusions and activation of Rac1 at the free edge. These results show a role for N-cadherin during contact inhibition of locomotion, and they reveal a mechanism of chemoattraction likely to function during both embryogenesis and cancer metastasis, whereby attractants such as Sdf1 amplify and stabilize contact-dependent cell polarity, resulting in directional collective migration. ► Neural crest (NC) cells undergo collective chemotaxis toward Sdf1 ► N-cadherin-dependent contacts are required for NC cell chemotaxis toward Sdf1 ► N-cadherin regulates contact inhibition of locomotion by controlling small GTPases ► Sdf1 reinforces cell polarity induced by N-cadherin/contact inhibition
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DOI：
10.1083/jcb.200812034
发表时间：
2009-06-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dupin I;Camand E;Etienne-Manneville S
通讯作者：
Etienne-Manneville S
影响因子：
5.3
作者：
Itoh, RE;Kurokawa, K;Matsuda, M
通讯作者：
Matsuda, M
影响因子：
3.4
作者：
Harms, BD;Bassi, GM;Lauffenburger, DA
通讯作者：
Lauffenburger, DA
DOI：
10.1073/pnas.252339399
发表时间：
2002-12-10
影响因子：
11.1
作者：
David, NB;Sapède, D;Ghysen, A
通讯作者：
Ghysen, A
影响因子：
2.7
作者：
Killian, Eugenia C. Olesnicky;Birkholz, Denise A.;Artinger, Kristin Bruk
通讯作者：
Artinger, Kristin Bruk