Elongator function in tRNA wobble uridine modification is conserved between yeast and plants
Elongator function in tRNA wobble uridine modification is conserved between yeast and plants
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tRNA 摆动尿苷修饰中的延伸子功能在酵母和植物之间是保守的
DOI：
10.1111/j.1365-2958.2010.07253.x
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发表时间：
2010
影响因子：
3.6
通讯作者：
Mehlgarten C
中科院分区：
文献类型：
--
作者：
Mehlgarten C
根据对酵母和哺乳动物细胞的研究，Elongator 复合物与组蛋白乙酰化、极化蛋白质运输和 tRNA 修饰等多种功能有关。在这里，我们发现缺乏 Elongator 亚基 AtELP3/ELO3 的拟南芥突变体在 tRNA 摆动尿苷修饰方面存在缺陷。此外，我们证明表达各自拟南芥Elongator同源物AtELP3/ELO3和AtELP1/ELO2的酵母elp3和delp1突变体组装完整的Elongator复合物，表明高度的结构保守性。令人惊讶的是，基于Elongator依赖的tRNA无义抑制和zymocin tRNase毒素测定的体内互补研究表明，虽然AtELP1挽救了酵母elp1突变体的缺陷，但最保守的Elongator基因AtELP3未能补充anelp3突变体。这种互补性的缺乏是由于与酵母ELP1不相容，因为anelp1 elp3酵母突变体中两种植物基因的共表达恢复了Elongator的体内tRNA修饰功能。类似地，AtELP1、notScELP1也支持酵母-植物Elp3杂种的部分互补，这表明AtElp1对Elp3的序列要求不如ScElp1严格。我们得出结论，酵母和植物 Elongator 具有 tRNA 修饰作用，并提出该功能可能在所有真核生物王国的 Elongator 中都保守。
Based on studies in yeast and mammalian cells the Elongator complex has been implicated in functions as diverse as histone acetylation, polarized protein trafficking and tRNA modification. Here we show thatArabidopsismutants lacking the Elongator subunit AtELP3/ELO3 have a defect in tRNA wobble uridine modification. Moreover, we demonstrate that yeastelp3andelp1mutants expressing the respectiveArabidopsisElongator homologuesAtELP3/ELO3andAtELP1/ELO2assemble integer Elongator complexes indicating a high degree of structural conservation. Surprisingly,in vivocomplementation studies based on Elongator‐dependent tRNA nonsense suppression and zymocin tRNase toxin assays indicated that whileAtELP1rescued defects of a yeastelp1mutant, the most conserved Elongator geneAtELP3, failed to complement anelp3mutant. This lack of complementation is due to incompatibility with yeastELP1as coexpression of both plant genes in anelp1 elp3yeast mutant restored Elongator's tRNA modification functionin vivo. Similarly,AtELP1, notScELP1also supported partial complementation by yeast–plant Elp3 hybrids suggesting that AtElp1 has less stringent sequence requirements for Elp3 than ScElp1. We conclude that yeast and plant Elongator share tRNA modification roles and propose that this function might be conserved in Elongator from all eukaryotic kingdoms of life.
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影响因子：
5.3
作者：
Johansson, Marcus J. O.;Esberg, Anders;Bystrom, Anders S.
通讯作者：
Bystrom, Anders S.
DOI：
10.1073/pnas.0913559107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Nelissen, Hilde;De Groeve, Steven;Van Lijsebettens, Mieke
通讯作者：
Van Lijsebettens, Mieke
影响因子：
2.6
作者：
Jablonowski, D;Fichtner, L;Schaffrath, R
通讯作者：
Schaffrath, R
影响因子：
9.8
作者：
Slaugenhaupt, SA;Blumenfeld, A;Gusella, JF
通讯作者：
Gusella, JF
影响因子：
1.6
作者：
Kishida, M;Tokunaga, M;Hishinuma, F
通讯作者：
Hishinuma, F