CO(2)-expanded nanofiber scaffolds maintain activity of encapsulated bioactive materials and promote cellular infiltration and positive host response.
CO(2)-expanded nanofiber scaffolds maintain activity of encapsulated bioactive materials and promote cellular infiltration and positive host response.
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DOI：
10.1016/j.actbio.2017.12.018
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发表时间：
2018-03-01
影响因子：
9.7
通讯作者：
Xie J
中科院分区：
文献类型：
--
作者：
Jiang J;Chen S;Wang H;Carlson MA;Gombart AF;Xie J
传统的电纺生物膜由于其固有的性质（例如，致密结构和小孔径）限制了它们在组织再生中的应用。在此，我们报告了一种简单而新颖的方法，通过亚临界CO2流体减压，将传统纳米纤维膜从二维扩展到具有受控厚度和孔隙率的三维（3D）。扩展的3D纳米支架形成分层结构，同时保持对齐的纳米形貌线索。三维支架还保留了包封的香豆素6的荧光强度和包封的抗菌肽LL-37的抗菌活性。此外，与传统的生物膜相比，具有阵列孔的扩展的3D生物膜支架可以显著促进皮下植入后的细胞浸润和新组织形成。与传统的生物膜相比，这种支架在皮下植入2周和4周后还显著增加了血管形成和M2/M1巨噬细胞的比率。总之，所提出的方法在制造用于各种应用的功能性3D生物相容性支架中具有巨大的潜力，包括工程化3D体外组织模型、抗菌伤口敷料和体内修复/再生组织。
Traditional electrospun nanofiber membranes were incapable of promoting cellular infiltration due to its intrinsic property (e.g., dense structure and small pore size) limiting their use in tissue regeneration. Herein, we report a simple and novel approach for expanding traditional nanofiber membranes from two-dimensional to three-dimensional (3D) with controlled thickness and porosity via depressurization of subcritical CO2 fluid. The expanded 3D nanofiber scaffolds formed layered structures and simultaneously maintained the aligned nanotopographic cues. The 3D scaffolds also retained the fluorescent intensity of encapsulated coumarin 6 and the antibacterial activity of encapsulated antimicrobial peptide LL-37. In addition, the expanded 3D nanofiber scaffolds with arrayed holes can significantly promote cellular infiltration and neotissue formation after subcutaneous implantation compared to traditional nanofiber membranes. Such scaffolds also significantly increased the blood vessel formation and the ratio of M2/M1 macrophages after subcutaneous implantation for 2 and 4 weeks compared to traditional nanofiber membranes. Together, the presented method holds great potential in the fabrication of functional 3D nanofiber scaffolds for various applications including engineering 3D in vitro tissue models, antimicrobial wound dressing, and repairing/regenerating tissues in vivo.
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DOI：
10.1016/j.msec.2013.08.010
发表时间：
2013-12-01
期刊：
Materials science & engineering. C, Materials for biological applications
影响因子：
--
作者：
Bhamidipati M;Sridharan B;Scurto AM;Detamore MS
通讯作者：
Detamore MS
DOI：
10.1073/pnas.1221403110
发表时间：
2013-04-16
影响因子：
11.1
作者：
Fong, Eliza Li Shan;Lamhamedi-Cherradi, Salah-Eddine;Ludwig, Joseph A.
通讯作者：
Ludwig, Joseph A.
影响因子：
3
作者：
Garland, Sandra M.;Menary, Robert C.
通讯作者：
Menary, Robert C.
影响因子：
4.1
作者：
Bonvallet, Paul P.;Culpepper, Bonnie K.;Bellis, Susan L.
通讯作者：
Bellis, Susan L.
影响因子：
14
作者：
Blakeney, Bryan A.;Tambralli, Ajay;Anderson, Joel M.;Andukuri, Adinarayana;Lim, Dong-Jin;Dean, Derrick R.;Jun, Ho-Wook
通讯作者：
Jun, Ho-Wook