A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus.
A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus.
复制标题
DOI：
10.1016/j.virol.2004.04.046
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发表时间：
2004-08-01
期刊：
影响因子：
3.7
通讯作者：
Pattnaik AK
中科院分区：
文献类型：
--
作者：
Truong HM;Lu Z;Kutish GF;Galeota J;Osorio FA;Pattnaik AK
测定了高致病性猪繁殖与呼吸综合征病毒（PRRSV）的核苷酸序列。用病毒基因组全长cDNA克隆的体外转录物转染MARC-145细胞，可获得生长特征与亲本病毒相似的传染性PRRSV。引物延伸分析显示，在复制过程中，病毒聚合酶纠正了转染转录物5 '端存在的两个非病毒鸟苷残基。动物研究表明，克隆病毒引起高热、持续性病毒血症和抗体反应，与亲本病毒相似。在将naïve猪引入接种了克隆病毒的猪群后3天内，接触传播迅速发生。这些结果表明，克隆病毒保留了亲本病毒的体内毒力和传染特性，从而为系统检查衰减和毒力表型的反向遗传学操作提供了背景。
The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that of the parental virus. Primer extension analysis revealed that during replication, the viral polymerase corrected the two nonviral guanosine residues present at the 5′ terminus of the transfected transcripts. Animal studies showed that the cloned virus induced hyperthermia, persistent viremia, and antibody response, similar to that observed with the parental virus. Contact transmission occurred rapidly within 3 days of introduction of naïve pigs into the group of clone virus-inoculated pigs. These results suggest that the cloned virus retains the in vivo virulence and contagion properties of the parental virus, thus, providing the background for reverse genetics manipulation in systematic examination of attenuation and virulence phenotypes.
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影响因子：
2.7
作者：
Allende R;Kutish GF;Laegreid W;Lu Z;Lewis TL;Rock DL;Friesen J;Galeota JA;Doster AR;Osorio FA
通讯作者：
Osorio FA
影响因子：
3.3
作者：
Mengeling, WL;Vorwald, AC;Brockmeier, SL
通讯作者：
Brockmeier, SL
影响因子：
3.8
作者：
HAYES, RJ;BUCK, KW
通讯作者：
BUCK, KW
影响因子：
3.8
作者：
Andrejeva, J;Puurand, Ü;Valkonen, JPT
通讯作者：
Valkonen, JPT
影响因子：
5.4
作者：
Meulenberg, JJM;BosDeRuijter, JNA;Moormann, RJM
通讯作者：
Moormann, RJM