Photoaffinity labeling identifies the substrate-binding site of mammalian squalene epoxidase.
Photoaffinity labeling identifies the substrate-binding site of mammalian squalene epoxidase.
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光亲和标记可识别哺乳动物角鲨烯环氧酶的底物结合位点。
DOI：
10.1016/j.bbrc.2004.01.012
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发表时间：
2004
影响因子：
3.1
通讯作者：
Prestwich,GlennD
中科院分区：
文献类型：
--
作者：
Lee,Hee-Kyoung;Zheng,YiFeng;Xiao,Xiao-Yi;Bai,Mei;Sakakibara,Jun;Ono,Teruo;Prestwich,GlennD
Squalene epoxidase (SE) catalyzes the conversion of squalene to (3S)-2,3-oxidosqualene. Photolabeling and site-directed mutagenesis were performed on recombinant rat SE (rrSE) in order to identify the location of the substrate-binding site and the roles of key residues in catalysis. Truncated 50-kDa rrSE was purified and photoaffinity labeled by competitive SE inhibitor (Ki=18.4μM), [3H]TNSA-Dza. An 8-kDa CNBr/BNPS-skatole peptide was purified and the first 24 amino acids were sequenced by Edman degradation. The sequence PASFLPPSSVNKRGVLLLGDAYNL corresponded to residues 388–411 of the full-length rat SE. Three nucleophilic residues (Lys-399, Arg-400, and Asp-407) were labeled by [3H]TNSA-Dza. Triple mutants were prepared in which bulky groups were used to replace the labeled charged residues. Purified mutant enzymes showed lower enzymatic activity and reduced photoaffinity labeling by [3H]TNSA-Dza. This constitutes the first evidence as to the identity of the substrate-binding site of SE.
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幼虫灯：十年来用保幼激素类似物进行光亲和标记。
影响因子：
3.8
作者：
G. Prestwich;K. Touhara;L. Riddiford;B. Hammock
通讯作者：
B. Hammock
DOI：
10.1016/s0021-9258(17)44716-8
发表时间：
1990-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Horie;Y. Tsuchiya;M. Hayashi;Y. Iida;Y. Iwasawa;Y. Nagata;Y. Sawasaki;H. Fukuzumi;K. Kitani;T. Kamei
通讯作者：
M. Horie;Y. Tsuchiya;M. Hayashi;Y. Iida;Y. Iwasawa;Y. Nagata;Y. Sawasaki;H. Fukuzumi;K. Kitani;T. Kamei
影响因子：
3.9
作者：
Laden, BP;Tang, YZ;Porter, TD
通讯作者：
Porter, TD
天然保幼激素 JH I、JH II 和 JH III 的同源氚标记光亲和类似物的合成
影响因子：
1.8
作者：
I. Ujváry;W. Eng;G. Prestwich
通讯作者：
G. Prestwich
哺乳动物角鲨烯环氧化酶的新型非角鲨烯类光亲和标签
影响因子：
2.7
作者：
M. Ceruso;G. Prestwich
通讯作者：
G. Prestwich