Gender-dependent miR-375 promoter methylation and the risk of type 2 diabetes.
Gender-dependent miR-375 promoter methylation and the risk of type 2 diabetes.
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性别依赖性 miR-375 启动子甲基化与 2 型糖尿病的风险
DOI：
10.3892/etm.2013.1069
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发表时间：
2013-06
影响因子：
2.7
通讯作者：
Duan S
中科院分区：
文献类型：
--
作者：
Cheng J;Wang L;Xu L;Wang H;Liu P;Bu S;Ye M;Zhang L;Wang Q;Duan S
启动子DNA甲基化可能反映了代谢紊乱（包括2型糖尿病（T2 D））发展中遗传背景和环境因素之间的相互作用。作为T2 D的表观遗传因子，miR-375在胰岛细胞的功能调节中发挥重要作用。在本研究中，我们研究了miR-375基因启动子DNA甲基化与T2 D风险的相关性。使用亚硫酸氢盐焦磷酸测序技术，在48例T2 D病例和48例健康对照中测量miR-375启动子上的8个CpG二核苷酸的DNA甲基化水平。女性中大多数CpG（CpG 7除外）的甲基化水平显著高于男性（P<0.05）。8个CpG的甲基化水平之间存在显著相关性（P<0.001）。miR-375基因启动子甲基化与T2 D风险之间无显著相关性（P=0.417）。在按性别进行的细分分析中观察到类似的结果（男性，P=0.844;女性，P=0.234）。此外，尽管在女性中发现miR-375的CpG 8甲基化水平与总甘油三酯水平之间存在相关性（P=0.009），但miR-375基因启动子中大多数CpG的DNA甲基化与个体的临床代谢特征无关。
Promoter DNA methylation may reflect the interaction between genetic background and environmental factors in the development of metabolic disorders, including type 2 diabetes (T2D). As an epigenetic factor of T2D, miR-375 plays an important role in the functional accommodation of islet cells. In the present study, we investigated the association of promoter DNA methylation of the miR-375 gene with the risk of T2D. Using bisulfite pyrosequencing technology, the DNA methylation levels of eight CpG dinucleotides on the miR-375 promoter were measured in 48 T2D cases and 48 healthy controls. The majority of CpGs (with the exception of CpG7) had significantly higher methylation levels in women compared with those in men (P<0.05). The methylation levels of the eight CpGs were significantly correlated with each other (P<0.001). No significant association between miR-375 gene promoter methylation and the risk of T2D was identified (P=0.417). Similar results were observed in the breakdown analysis by gender (men, P=0.844; women, P=0.234). In addition, although a correlation between the CpG8 methylation level of miR-375 and total triglyceride level was identified in women (P=0.009), DNA methylation of the majority of CpGs in the miR-375 gene promoter was not associated with the clinical metabolic features of the individuals.
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影响因子：
7.7
作者：
Fernandez-Valverde SL;Taft RJ;Mattick JS
通讯作者：
Mattick JS
影响因子：
3.7
作者：
Husseiny MI;Kuroda A;Kaye AN;Nair I;Kandeel F;Ferreri K
通讯作者：
Ferreri K
影响因子：
11.4
作者：
Volkmar, Michael;Dedeurwaerder, Sarah;Cunha, Daniel A.;Ndlovu, Matladi N.;Defrance, Matthieu;Deplus, Rachel;Calonne, Emilie;Volkmar, Ute;Igoillo-Esteve, Mariana;Naamane, Najib;Del Guerra, Silvia;Masini, Matilde;Bugliani, Marco;Marchetti, Piero;Cnop, Miriam;Eizirik, Decio L.;Fuks, Francois
通讯作者：
Fuks, Francois
影响因子：
64.5
作者：
LEE, RC;FEINBAUM, RL;AMBROS, V
通讯作者：
AMBROS, V
影响因子：
3.5
作者：
Li, Lian;Lee, Kyoung-Mu;Kang, Daehee
通讯作者：
Kang, Daehee