Genotyping human papillomavirus type 16 isolates from persistently infected promiscuous individuals and cervical neoplasia patients
Genotyping human papillomavirus type 16 isolates from persistently infected promiscuous individuals and cervical neoplasia patients
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对持续感染的混杂个体和宫颈肿瘤患者中分离出的人乳头瘤病毒 16 型进行基因分型
DOI：
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发表时间：
1995
影响因子：
9.4
通讯作者：
W. Quint
中科院分区：
文献类型：
--
作者：
A. Belkum;L. Juffermans;Lianne Schrauwen;G. V. Doornum;M. Burger;W. Quint
Nucleotide sequence variation in the noncoding region of the genome of human papillomavirus type 16 (HPV16) was determined by direct sequencing and single-strand conformation polymorphism analysis of DNA fragments amplified by PCR. Individuals of diverse sexual promiscuity and/or cervicopathology were studied. In a group of 14 healthy, monogamous HPV16-positive females, only two HPV16 sequence variants could be documented. Among 17 females and 3 males with multiple sex partners and living in the same geographical region, nine sequence variants were found, whereas among 7 patients with cervical neoplasia from another region, five variants were detected. Although numbers are limited, in the group of individuals at high risk of acquiring a sexually transmitted disease or with cervical neoplasia, a larger number of HPV16 sequence variants was encountered (two types among 14 individuals versus nine types among 20; Fisher's exact test, P = 0.07). Seven of the individuals were sampled repeatedly over time. For these persistently infected women, no differences in HPV16 sequences were detected, irrespective of promiscuity, and persistence of a single viral variant, spread over multiple anatomic sites, for more than 2 years could be demonstrated. This indicates that viral persistence may be a common feature and that successful superinfection with a new variant may be rare, despite a potentially high frequency of viral reinoculation.
人乳头瘤病毒 6 型和 16 型 L2 蛋白两个免疫显性区域的序列和抗原多样性。
DOI：
10.1093/infdis/168.3.743
发表时间：
1993
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Yaegashi,N;Xi,L;Batra,M;Galloway,DA
通讯作者：
Galloway,DA
单链构象多态性分析检测人乳头瘤病毒16型非编码区序列变异。
DOI：
10.1093/infdis/168.3.610
发表时间：
1993
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Xi,LF;Demers,W;Kiviat,NB;Kuypers,J;Beckmann,AM;Galloway,DA
通讯作者：
Galloway,DA
免疫抑制患者尖锐湿疣中多种人乳头瘤病毒类型的检测。
DOI：
10.1093/infdis/170.4.759
发表时间：
1994
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Brown,DR;Bryan,JT;Cramer,H;Katz,BP;Handy,V;Fife,KH
通讯作者：
Fife,KH