Reticular Basement Membrane Thickness Is Associated with Growth- and Fibrosis-Promoting Airway Transcriptome Profile-Study in Asthma Patients.
Reticular Basement Membrane Thickness Is Associated with Growth- and Fibrosis-Promoting Airway Transcriptome Profile-Study in Asthma Patients.
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网状地下膜厚度与哮喘患者中促进生长和纤维化的气道转录组概况研究有关。
DOI：
10.3390/ijms22030998
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发表时间：
2021-01-20
影响因子：
5.6
通讯作者：
Bazan JG
中科院分区：
文献类型：
--
作者：
Bazan-Socha S;Buregwa-Czuma S;Jakiela B;Zareba L;Zawlik I;Myszka A;Soja J;Okon K;Zarychta J;Kozlik P;Dziedzina S;Padjas A;Wojcik K;Kepski M;Bazan JG
Airway remodeling in asthma is characterized by reticular basement membrane (RBM) thickening, likely related to epithelial structural and functional changes. Gene expression profiling of the airway epithelium might identify genes involved in bronchial structural alterations. We analyzed bronchial wall geometry (computed tomography (CT)), RBM thickness (histology), and the bronchial epithelium transcriptome profile (gene expression array) in moderate to severe persistent (n = 21) vs. no persistent (n = 19) airflow limitation asthmatics. RBM thickness was similar in the two studied subgroups. Among the genes associated with increased RBM thickness, the most essential were those engaged in cell activation, proliferation, and growth (e.g., CDK20, TACC2, ORC5, and NEK5) and inhibiting apoptosis (e.g., higher mRNA expression of RFN34, BIRC3, NAA16, and lower of RNF13, MRPL37, CACNA1G). Additionally, RBM thickness correlated with the expression of genes encoding extracellular matrix (ECM) components (LAMA3, USH2A), involved in ECM remodeling (LTBP1), neovascularization (FGD5, HPRT1), nerve functioning (TPH1, PCDHGC4), oxidative stress adaptation (RIT1, HSP90AB1), epigenetic modifications (OLMALINC, DNMT3A), and the innate immune response (STAP1, OAS2). Cluster analysis revealed that genes linked with RBM thickness were also related to thicker bronchial walls in CT. Our study suggests that the pro-fibrotic profile in the airway epithelial cell transcriptome is associated with a thicker RBM, and thus, may contribute to asthma airway remodeling.
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影响因子：
64.8
作者：
Fimia, Gian Maria;Stoykova, Anastassia;Cecconi, Francesco
通讯作者：
Cecconi, Francesco
影响因子：
5.3
作者：
Alpatov, R;Munguba, GC;Sugrue, SP
通讯作者：
Sugrue, SP
影响因子：
11.4
作者：
Carretero, Maria;Ruiz-Torres, Miguel;Losada, Ana
通讯作者：
Losada, Ana
影响因子：
4.7
作者：
Khurana N;Bhattacharyya S
通讯作者：
Bhattacharyya S
DOI：
10.1083/jcb.200210005
发表时间：
2003-01-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goshima G;Kiyomitsu T;Yoda K;Yanagida M
通讯作者：
Yanagida M