Type V collagen in experimental granulation tissue.
Type V collagen in experimental granulation tissue.
复制标题
实验肉芽组织中的 V 型胶原蛋白。
作者：
K. Inkinen;H. Wolff;K. von Boguslawski;J. Ahonen
为了评估创伤愈合模型中V型胶原的空间和时间表达，使用大鼠皮下植入的粘胶纤维素海绵来诱导肉芽组织形成。在第3、5、8、14、21、30、59和84天对肉芽组织进行分析。提取酸溶性胶原，并通过SDS-PAGE定量V型胶原的相对量。免疫组化中使用I、III和V型胶原的特异性抗体，原位杂交中使用proalpha 1（I）、proalpha 1（III）和proalpha 1（V）胶原的特异性RNA探针。V型胶原蛋白含量相对于I型和III型胶原蛋白增加至第8天，并保持在相同的水平长达三个月。免疫组化显示血管壁中V型胶原表达强烈。原位杂交结果显示，三种类型的胶原蛋白均主要表达于成纤维细胞样细胞，圆形细胞中也有表达，尤其是V型胶原。总之，从第3天开始，在伤口愈合模型中观察到数量增加的V型胶原，其定位与肉芽组织中的血管高度相关，并且由成纤维细胞样和圆形细胞合成。
To evaluate the spatial and temporal expression of type V collagen in a wound healing model, subcutaneously implanted viscose cellulose sponges in rats were used to induce granulation tissue formation. Analyses on granulation tissue were carried out on days 3, 5, 8, 14, 21, 30, 59 and 84. Acid soluble collagens were extracted and the relative amount of type V collagen was quantified by SDS-PAGE. Specific antibodies to type I, III and V collagens were used in immunohistochemistry and specific RNA probes to proalpha1(I), proalpha1(III) and proalpha1(V) collagen in in situ hybridization. Type V collagen content increased relative to type I and III collagens up to day 8 and remained at the same level for up to the three months. Type V collagen was expressed strongly in blood vessel walls as seen in immunohistochemistry. In situ hybridization showed that all of the three types of collagen were expressed mostly in fibroblast-like cells and also in rounded cells, especially type V collagen. In conclusion, type V collagen was seen in the wound healing model in increasing amounts from day 3 onwards, its localization being highly associated with blood vessels in granulation tissue and it was synthesized by fibroblast-like and rounded cells.
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DOI：
--
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影响因子：
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DOI：
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DOI：
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