Comparison of RNAscope and immunohistochemistry for evaluation of the UPK2 status in urothelial carcinoma tissues.
Comparison of RNAscope and immunohistochemistry for evaluation of the UPK2 status in urothelial carcinoma tissues.
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RNAscope和免疫组织化学评估尿路上皮癌组织中UPK2状态的比较
DOI：
10.1186/s13000-022-01191-x
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发表时间：
2022-01-14
影响因子：
2.6
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Lu J;Zhao M;Wu C;Chu C;Zhang CZ;Cao Y
UPK2对尿路上皮癌（UC）具有良好的特异性。UPK2的评价有助于UC的正确诊断。然而，通过免疫组织化学（IHC）检测UPK2的灵敏度相对较低。本文旨在比较RNAscope和IHC在UC中评估UPK2状态的诊断灵敏度。本研究选择了127例常规膀胱UC、45例变异膀胱UC、24例上尿路UC和23例转移性UC的组织块。采用免疫组化和RNAscope法检测UCs中UPK2的表达情况。然后，对两种方法进行了比较。RNAscope和IHC对UC中UPK2阳性率的评价无显著差异（68.0% vs. 62.6%，P = 0.141）。相关性分析显示UPK2检测的中度正相关：RNAscope vs. IHC（P < 0.001，R = 0.441）。我们的研究结果显示，在变异型膀胱UC中，RNAscope检测到的UPK2阳性率（53.3%）高于IHC检测到的阳性率（35.6%）。令人遗憾的是，P值未显示显著差异（P = 0.057）。UPK2的RNAscope表现与IHC相似，阳性率略高，表明其可用作UPK2 IHC的替代或辅助。
UPK2 exhibits excellent specificity for urothelial carcinoma (UC). UPK2 evaluation can be useful in making the correct diagnosis of UC. However, UPK2 detection by immunohistochemistry (IHC) has relatively low sensitivity. This paper aimed to compare the diagnostic sensitivity of RNAscope and IHC for evaluation of the UPK2 status in UC. Tissue blocks from 127 conventional bladder UCs, 45 variant bladder UCs, 24 upper tract UCs and 23 metastatic UCs were selected for this study. IHC and RNAscope were used to detect the UPK2 status in UCs. Then, comparisons of the two methods were undertaken. There was no significant difference between RNAscope and IHC for the evaluation of the UPK2 positivity rate in UC (68.0% vs. 62.6%, P = 0.141). Correlation analysis revealed a moderate positive correlation for detection of UPK2: RNAscope vs. IHC (P < 0.001, R = 0.441). Our results showed a trend toward a higher positive UPK2 rate detected by RNAscope (53.3%) than by IHC (35.6%) in variant bladder UCs. Disappointingly, the P value did not indicate a significant difference (P = 0.057). RNAscope for UPK2 appeared to perform similarly to IHC, with a marginally higher positive rate, suggesting it could be used as an alternative or adjunct to UPK2 IHC.
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影响因子：
19.6
作者：
Wu, Xue-Ru;Kong, Xiang-Peng;Pellicer, Angel;Kreibich, Gert;Sun, Tung-Tien
通讯作者：
Sun, Tung-Tien
影响因子：
4.6
作者：
Aine M;Eriksson P;Liedberg F;Sjödahl G;Höglund M
通讯作者：
Höglund M
影响因子：
3.3
作者：
Tian, Wei;Guner, Gunes;Netto, George J.
通讯作者：
Netto, George J.
影响因子：
11.1
作者：
Dadhania, Vipulkumar;Zhang, Miao;Zhang, Li;Bondaruk, Jolanta;Majewski, Tadeusz;Siefker-Radtke, Arlene;Guo, Charles C.;Dinney, Colin;Cogdell, David E.;Zhang, Shizhen;Lee, Sangkyou;Lee, June G.;Weinstein, John N.;Baggerly, Keith;McConkey, David;Czerniak, Bogdan
通讯作者：
Czerniak, Bogdan
影响因子：
6.6
作者：
Li, SM;Zhang, ZT;Wu, XR
通讯作者：
Wu, XR