MALDI imaging of lipid biochemistry in tissues by mass spectrometry.
MALDI imaging of lipid biochemistry in tissues by mass spectrometry.
复制标题
通过质谱法对组织中脂质生物化学的MALDI成像。
DOI：
10.1021/cr200280p
复制
发表时间：
2011-10-12
期刊：
影响因子：
62.1
通讯作者：
Murphy, Robert C.
中科院分区：
文献类型：
--
作者：
Berry, Karin A. Zemski;Hankin, Joseph A.;Barkley, Robert M.;Spraggins, Jeffrey M.;Caprioli, Richard M.;Murphy, Robert C.
由于最近的进展，显着的图像显示复杂的脂质在组织中的分布，现在产生的基质辅助激光解吸/电离成像质谱（MALDI IMS）。脂质是具有疏水结构特征的两亲性生物分子，其通过初始阴离子硫酯缩合反应（脂肪酸合酶）或通过支链焦磷酸中间体的碳阳离子缩合（异戊二烯途径）制成。1脂质在细胞功能中发挥重要作用，包括磷脂的自组装，以形成每个活细胞的组成性外膜和内膜双层。这些膜磷脂的特定组分包括含有酯化花生四烯酸酯的物质，所述酯化花生四烯酸酯可以酶促释放为游离酸并转化为具有无数生物学效应的有效信号分子（异黄酮、白三烯）。脂质胆固醇是双层膜的重要组成部分，具有复杂但高度调控的生物合成。血液中胆固醇水平（主要是胆固醇酯）的升高与心脏病有关，通常出于人类健康的考虑进行监测。一些脂质分子在生物化学能量储存中起着核心作用，其形式为三酰甘油分子，储存在几乎所有细胞内的脂质体中。质谱法历来是脂质生物化学研究的首选工具。质谱数据的灵敏度和特异性有助于理清脂质结构的复杂性，从而开始跟踪生物学变化。虽然荧光共聚焦显微镜等技术或利用荧光标签设计可在细胞中表达的蛋白质的能力已成为现代生物化学研究的主流，但由于脂质分子的相对较小尺寸及其在细胞中结构的动态性质，此类技术不适用于大多数脂质。MALDI IMS的最新发展已经将脂质鉴定的特异性与二维分子图谱结合起来，从而能够对生物组织的各个区域进行脂质的生化研究。
As a result of recent advances, remarkable images revealing the distribution of complex lipids in tissues are now generated by matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS). Lipids are amphipathic biomolecules with hydrophobic structural characteristics made by either an initial anion thioester condensation reaction (fatty acid synthase) or by carbocation condensation of branched-chain pyrophosphate intermediates (isoprene pathway). 1 Lipids play essential roles in cellular function including the self-assembly of phospholipids to form the constitutive outer and inner membrane bilayer of every living cell. Specific components of these membrane phospholipids include species that contain esterified arachidonate that can be enzymatically released to a free acid and transformed to potent signaling molecules (prostaglandins, leukotrienes) with myriad biological effects. The lipid cholesterol is an essential component of bilayer membranes that has a complicated, yet highly regulated biosynthesis. Elevation of cholesterol levels (predominantly as cholesteryl esters) in blood has been implicated in heart disease and is commonly monitored in consideration of human health. Some lipid molecules play the central role in biochemical energy storage in the form of triacylglycerol molecules stored in lipid bodies within almost all cells.Mass spectrometry has historically been a tool of choice in biochemical studies of lipids. The sensitivity and specificity of mass spectral data are useful to sort out the complexity of lipid structures to begin to follow biological changes. While techniques such as fluorescence confocal microscopy or the ability to engineer proteins that can be expressed in cells with fluorescent tags have become the mainstream of modern biochemical research, such techniques are not amenable to most lipids because of the relatively small size of the lipid molecules and the dynamic nature of their structure in the cell. Recent developments in MALDI IMS have merged specificity of lipid identification with two-dimensional molecular mapping to enable biochemical studies of lipids across regions of a biological tissue.
登录
查看更多内容
DOI：
10.1016/j.jasms.2009.01.017
发表时间：
2009-06
影响因子：
3.2
作者：
Chen R;Hui L;Sturm RM;Li L
通讯作者：
Li L
影响因子：
7.4
作者：
Baluya, Dodge L.;Garrett, Timothy J.;Yost, Richard A.
通讯作者：
Yost, Richard A.
影响因子：
6.5
作者：
Berry, Karin A. Zemski;Li, Bilan;Murphy, Robert C.
通讯作者：
Murphy, Robert C.
影响因子：
62.1
作者：
Chughtai, Kamila;Heeren, Ron M. A.
通讯作者：
Heeren, Ron M. A.
DOI：
10.1007/s13361-011-0172-2
发表时间：
2011-09
影响因子：
3.2
作者：
Castro-Perez, Jose;Roddy, Thomas P.;Nibbering, Nico M. M.;Shah, Vinit;McLaren, David G.;Previs, Stephen;Attygalle, Athula B.;Herath, Kithsiri;Chen, Zhu;Wang, Sheng-Ping;Mitnaul, Lyndon;Hubbard, Brian K.;Vreeken, Rob J.;Johns, Douglas G.;Hankemeier, Thomas
通讯作者：
Hankemeier, Thomas