Detection of abnormal peripheral blood mononuclear cell cytokine networks in human IgA nephropathy.
Detection of abnormal peripheral blood mononuclear cell cytokine networks in human IgA nephropathy.
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人 IgA 肾病外周血单核细胞细胞因子网络异常的检测。
DOI：
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发表时间：
1993
影响因子：
19.6
通讯作者：
Francis W. Ballardie
中科院分区：
文献类型：
--
作者：
Mark P. de Caestecker;Martin Bottomley;Brian A. Telfer;Ian V. Hutchinson;Brent M. Vose;Francis W. Ballardie
细胞因子表达异常参与了IgA肾病的发病机制，但其机制和选择性尚不清楚。在这项研究中，我们检测了45例IgA肾病患者外周血单个核细胞(PBMNC)一系列免疫调节细胞因子mRNAs的表达，这些患者根据尿红细胞排泄量进行经验分层：10例缓解期，35例活动期(21例轻度，14例中度)，17例正常对照和15例疾病对照。我们使用了半定量聚合酶链式反应(PCR)技术。所有患者近期均未出现肉眼血尿。同时用免疫荧光双色细胞仪检测单核细胞II类抗原(DR)的表达。转化生长因子-β1mRNA在活动期和非活动期IgA肾病患者中分别为68%(24/35)和70%(7/10)，而在正常对照组(P<；0.005)和对照组中分别为18%(3/17)和27%(4/15)。活动期IgA肾病患者IL-6转录本的阳性率为37%(13/35)，而正常对照组为6%(1/17)(P=0.015)，且IgA缓解组和疾病对照组IL-6转录本的表达均无明显增加。正常对照组和疾病对照组分别为29%(5/17)和53%(8/15)，而IgA肾病组仅7%(3/35)(P=0.015)。转化生长因子-β2、干扰素、IL-2、IL-4、IL-1α、IL-1β检测结果组间差异无统计学意义。
Dysregulated cytokine expression has been implicated in the pathogenesis of IgA nephropathy, but the mechanisms and selectivity of this response are poorly understood. In this study we have examined the expression of a range of immunoregulatory cytokine mRNAs by peripheral blood mononuclear cells (PBMNCs) from 45 patients with IgA nephropathy stratified empirically according to urinary red cell excretion: 10 in remission, and 35 with active disease (21 mild, 14 moderate), and 17 normal, and 15 disease, controls. We used a semi-quantitative polymerase chain reaction (PCR) technique. None of the patients had experienced recent episodes of macroscopic hematuria. Simultaneous analysis of monocyte class II antigen (DR) expression was also performed by two-color immunoflow cytometry. TGF-beta 1 mRNA was detected in 68% (24 of 35) of patients with active, and 70% (7 of 10) inactive IgA nephropathy, but in only 18% (3 of 17) normal (P < 0.005), and 27% (4 of 15) disease controls. IL-6 transcripts were identified in 37% (13 of 35) of patients with active IgA nephropathy, compared with 6% (1 of 17) normal controls (P = 0.015), with no significant increase in IgA remission, or disease control groups. TNF-alpha mRNA was detected in 29% (5 of 17) of normal and 53% (8 of 15) disease controls, but in only 7% (3 of 35) of patients with IgA nephropathy (P = 0.015). There was no significant change in TGF-beta 2, gamma-IFN, IL-2, IL-4, IL-1 alpha or IL-1 beta detection between groups.(ABSTRACT TRUNCATED AT 250 WORDS)
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影响因子：
15.9
作者：
HOLDSWORTH, SR;NEALE, TJ;WILSON, CB
通讯作者：
WILSON, CB
影响因子：
19.6
作者：
RUEF, C;BUDDE, K;COLEMAN, DL
通讯作者：
COLEMAN, DL
影响因子：
4.4
作者：
M. Linker‐Israeli;R. Deans;D. Wallace;J. Prehn;T. Ozeri-Chen;J. Klinenberg
通讯作者：
M. Linker‐Israeli;R. Deans;D. Wallace;J. Prehn;T. Ozeri-Chen;J. Klinenberg
DOI：
10.1073/pnas.87.7.2725
发表时间：
1990-04-01
影响因子：
11.1
作者：
GILLILAND, G;PERRIN, S;BUNN, HF
通讯作者：
BUNN, HF
影响因子：
4.4
作者：
G. Firestein;J. Álvaro-Gracia;R. Maki
通讯作者：
G. Firestein;J. Álvaro-Gracia;R. Maki