Lipopolysaccharide from Porphyromonas gingivalis sensitizes capsaicin-sensitive nociceptors.
Lipopolysaccharide from Porphyromonas gingivalis sensitizes capsaicin-sensitive nociceptors.
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DOI：
10.1016/j.joen.2007.07.001
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发表时间：
2011-01
影响因子：
4.2
通讯作者：
Diogenes, Anibal
中科院分区：
文献类型：
--
作者：
Randi Ferraz, Caio Cezar;Henry, Michael A.;Hargreaves, Kenneth M.;Diogenes, Anibal
虽然牙源性感染常伴有疼痛，但对介导这种效应的潜在机制知之甚少。在这项研究中，我们测试的假设，三叉神经伤害性神经元直接致敏的脂多糖（LPS）分离牙髓病原体，牙龈卟啉单胞菌（牙龈卟啉单胞菌）。用大鼠三叉神经元培养物进行的体外研究表明，与溶剂预处理相比，用LPS预处理产生辣椒素诱发的降钙素基因相关肽（CGRP）释放的显著增加，从而显示辣椒素受体TRPV 1由LPS致敏。此外，人牙髓样品的共聚焦显微镜检查显示LPS受体（toll样受体4; TLR 4）与含有CGRP的神经纤维共定位。总的来说，这些结果表明，在感染的管道系统中发现的浓度的LPS对伤害感受器的直接致敏是与细菌感染相关的疼痛的一种机制。
Although odontogenic infections are often accompanied by pain, little is known about the potential mechanisms mediating this effect. In this study, we tested the hypothesis that trigeminal nociceptive neurons are directly sensitized by lipopolysaccharide (LPS) isolated from an endodontic pathogen, Porphyromonas gingivalis (P. gingivalis). In vitro studies conducted with cultures of rat trigeminal neurons demonstrated that pretreatment with LPS produced a significant increase in the capsaicin-evoked release of calcitonin gene-related peptide (CGRP) when compared to vehicle pretreatment, thus showing sensitization of the capsaicin receptor, TRPV1, by LPS. Furthermore, confocal microscopic examination of human tooth pulp samples showed the colocalization of the LPS receptor (toll-like receptor 4; TLR4) with CGRP containing nerve fibers. Collectively, these results suggest the direct sensitization of nociceptors by LPS at concentrations found in infected canal systems as one mechanism responsible for the pain associated with bacterial infections.
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影响因子：
4.2
作者：
Hargreaves, KM;Jackson, DL;Bowles, WR
通讯作者：
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影响因子：
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