Characterization of a pESI-like plasmid and analysis of multidrug-resistant Salmonella enterica Infantis isolates in England and Wales.
Characterization of a pESI-like plasmid and analysis of multidrug-resistant Salmonella enterica Infantis isolates in England and Wales.
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DOI：
10.1099/mgen.0.000658
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发表时间：
2021-10
影响因子：
3.9
通讯作者：
Nair S
中科院分区：
文献类型：
--
作者：
Lee WWY;Mattock J;Greig DR;Langridge GC;Baker D;Bloomfield S;Mather AE;Wain JR;Edwards AM;Hartman H;Dallman TJ;Chattaway MA;Nair S
肠道沙门氏菌 婴儿血清型是第五常见的 沙门 在英格兰和威尔士分离的血清型。流行病学、基因分型和抗菌药物耐药性数据 S . enterica Infantis分离株被用来分析英语和威尔士人口超过5年的时间。与下列人员有关的旅行案件 S . enterica 婴儿主要来自亚洲，其次是欧洲和北美的病例。自2000年以来， S . enterica 婴儿的多药耐药决定簇存在于一个大的质粒上，称为"新兴质粒“。 S . enterica Infantis'（pESI）。2013年至2018年，42 S . enterica 婴儿分离株从人类和食物中分离，这些人和食物对pESI样质粒上存在的多种抗菌剂类别具有耐药性决定簇，包括超广谱β-内酰胺酶（ESBLs; bla CTX-M-65）。产ESBL人的纳米孔测序 S . enterica Infantis分离株表明IncFIB pESI样质粒上存在两个区域，其中含有多个耐药基因。英格兰人和威尔士人的系统发育分析 S . enterica Infantis群体表明，大多数携带pESI样质粒的多重耐药分离株属于群体内维持的单一进化枝。2013年首次分离的bla CTX-M-65 ESBL分离株包含该进化枝内的一个谱系，主要与南美洲相关。因此，我们的数据显示，出现了一个稳定的耐药克隆，已经在英格兰和威尔士的人群中流通了一段时间，突出了监测这种血清型耐药性的必要性。
Salmonella enterica serovar Infantis is the fifth most common Salmonella serovar isolated in England and Wales. Epidemiological, genotyping and antimicrobial-resistance data for S . enterica Infantis isolates were used to analyse English and Welsh demographics over a 5 year period. Travel cases associated with S . enterica Infantis were mainly from Asia, followed by cases from Europe and North America. Since 2000, increasing numbers of S . enterica Infantis had multidrug resistance determinants harboured on a large plasmid termed ‘plasmid of emerging S . enterica Infantis’ (pESI). Between 2013 and 2018, 42 S . enterica Infantis isolates were isolated from humans and food that harboured resistance determinants to multiple antimicrobial classes present on a pESI-like plasmid, including extended-spectrum β-lactamases (ESBLs; bla CTX-M-65). Nanopore sequencing of an ESBL-producing human S . enterica Infantis isolate indicated the presence of two regions on an IncFIB pESI-like plasmid harbouring multiple resistance genes. Phylogenetic analysis of the English and Welsh S . enterica Infantis population indicated that the majority of multidrug-resistant isolates harbouring the pESI-like plasmid belonged to a single clade maintained within the population. The bla CTX-M-65 ESBL isolates first isolated in 2013 comprise a lineage within this clade, which was mainly associated with South America. Our data, therefore, show the emergence of a stable resistant clone that has been in circulation for some time in the human population in England and Wales, highlighting the necessity of monitoring resistance in this serovar.
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影响因子：
3.9
作者：
Connor TR;Loman NJ;Thompson S;Smith A;Southgate J;Poplawski R;Bull MJ;Richardson E;Ismail M;Thompson SE;Kitchen C;Guest M;Bakke M;Sheppard SK;Pallen MJ
通讯作者：
Pallen MJ
DOI：
10.1093/bioinformatics/btx610
发表时间：
2018-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Hadfield J;Croucher NJ;Goater RJ;Abudahab K;Aanensen DM;Harris SR
通讯作者：
Harris SR
影响因子：
5.8
作者：
Dallman, Timothy;Ashton, Philip;Grant, Kathie
通讯作者：
Grant, Kathie
影响因子：
2.6
作者：
Gilmour, MW;Thomson, NR;Taylor, DE
通讯作者：
Taylor, DE
影响因子：
11.8
作者：
Gal-Mor O;Valinsky L;Weinberger M;Guy S;Jaffe J;Schorr YI;Raisfeld A;Agmon V;Nissan I
通讯作者：
Nissan I