Exposure of the major human red-cell glycolipid, globoside, to galactose oxidase.

Exposure of the major human red-cell glycolipid, globoside, to galactose oxidase.
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主要的人红细胞糖脂、球苷脂暴露于半乳糖氧化酶。

DOI:
10.1111/j.1432-1033.1984.tb08524.x
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发表时间:
1984
期刊:
European journal of biochemistry
影响因子:
--
通讯作者:
Gahmberg,CG
Gahmberg,CG
中科院分区:
--
文献类型:
--
作者:
Lampio,A;Finne,J;Homer,D;Gahmberg,CG

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Plasma membrane glycolipids are localized at the outer leaflet of the lipid bilayer, and their carbohydrate portions are exposed to the environment. The efficiency of exposure has, however, not been known. We have been able to determine the availability of the major red cell glycolipid, globoside, to externally added galactose oxidase. Red cells were extensively treated with the enzyme and the oxidized cells reduced with NaBD4. After isolation the extent of exposed globoside was estimated by mass spectrometry. The results show that the exposure of globoside varies in red cells of different individuals from 37–66%. The fatty acid composition of externally available globoside was the same as that of non‐oxidized globoside. The exposure was not influenced by protease treatment of intact cells and no correlation was found with different ABO blood groups.
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