Illuminating type collections of nectriaceous fungi in Saccardo's fungarium.

Illuminating type collections of nectriaceous fungi in Saccardo's fungarium.
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DOI:
10.3767/persoonia.2020.45.09
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发表时间:
2020-12
期刊:
影响因子:
9.1
通讯作者:
Baldan B
Baldan B
中科院分区:
生物学1区
文献类型:
--
作者:
Forin N;Vizzini A;Nigris S;Ercole E;Voyron S;Girlanda M;Baldan B

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Nectria spp.的标本。和褐纹藻Nectriella rufofusca,通过基于MiSeq技术的形态和分子生物学方法对其进行了研究。从24个‘Nectria’Sensu Saccardo标本(包括20个类型)和模式标本中成功地获得了ITS1和ITS2序列。对于歧义线虫、弧度线虫和新月形线虫,仅恢复了ITS1序列。在形态和分子分析的基础上,提出了新的命名组合:白毛莲Nectria albofimbriata,Nectria albofimbriata,N.Ambiua var.Pallens、细粒拟青霉、小青霉亚种。Reyesiana、N.Radians、N.Squamuligera、N.tjibodensis和N.Constera、N.flageolitiana、N.Phyllostachydis、N.sordescens和N.tjibodensis var.提出了火烧菌的新概念。此外,目前的分类被确认为Nectria coronata、N.cyanostoma、N.dolichospora、N.illudens、N.leucotricha、N.mantuana、N.raripia和Nectriella rufofusca。这是第一次对这些100多年前的标本进行分子分析,从而为这些名称提供了重要的新的DNA序列数据。
Specimens of Nectria spp. and Nectriella rufofusca were obtained from the fungarium of Pier Andrea Saccardo, and investigated via a morphological and molecular approach based on MiSeq technology. ITS1 and ITS2 sequences were successfully obtained from 24 specimens identified as ‘Nectria’ sensu Saccardo (including 20 types) and from the type specimen of Nectriella rufofusca. For Nectria ambigua, N. radians and N. tjibodensis only the ITS1 sequence was recovered. On the basis of morphological and molecular analyses new nomenclatural combinations for Nectria albofimbriata, N. ambigua, N. ambigua var. pallens, N. granuligera, N. peziza subsp. reyesiana, N. radians, N. squamuligera, N. tjibodensis and new synonymies for N. congesta, N. flageoletiana, N. phyllostachydis, N. sordescens and N. tjibodensis var. crebrior are proposed. Furthermore, the current classification is confirmed for Nectria coronata, N. cyanostoma, N. dolichospora, N. illudens, N. leucotricha, N. mantuana, N. raripila and Nectriella rufofusca. This is the first time that these more than 100-yr-old specimens are subjected to molecular analysis, thereby providing important new DNA sequence data authentic for these names.
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