Allergens in Aspergillus fumigatus
Allergens in Aspergillus fumigatus
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烟曲霉中的过敏原
DOI：
10.1111/j.1398-9995.1994.tb02655.x
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发表时间：
1994
期刊：
影响因子：
12.4
通讯作者：
A. Karlsson
中科院分区：
文献类型：
--
作者：
M. Hansen;J. Wold;B. Paulsen;E. H. Cohen;A. Karlsson
提取在浸没培养物和表面培养物中生长的烟曲霉，并分别分析提取物。浸提液含蛋白质31.9%，碳水化合物8.3%，而相应的值为17.0%和33.3%的表面材料。对于过敏性哮喘患者的个体血清，SDS-PAGE结合免疫印迹显示，除了几种弱至中等IgE结合组分外，浸没提取物还含有至少六种强IgE结合组分（20、30、38、50、68和90 kDa）。表面提取物含有大约相同数量的IgE结合组分，但只有一种产生强烈反应（20 kDa）。通过等电聚焦（IEF）结合免疫印迹法证明，存在的过敏原的pI在4.5和5.6之间。为了鉴定A.烟曲霉糖蛋白过敏原，两种提取物在温和条件下用高碘酸盐处理。浸没提取物的两种过敏原（90和38 kDa）通过该处理部分丧失了其IgE结合能力，表明这些组分是糖蛋白，并且碳水化合物部分参与IgE结合。高碘酸盐不影响20 kDa过敏原的IgE结合能力。为了评估两种过敏原提取物的稳定性，将水溶液在4°C下保持2、7和21天，然后通过SDS-PAGE和免疫印迹进行分析。结果表明，浸提液中的大部分变应原在2d后被部分灭活。21天后，只有20-kDa和30-kDa组分仍然能够结合IgE。通过分析表面提取物获得了类似的结果。当在50%甘油溶液中对样品进行相同的实验时，结果强烈表明甘油对两种提取物中的过敏原具有稳定作用。通过API ZYM试验估计的酶含量表明，两种浸提液均含有几种蛋白质和碳水化合物降解酶。这些酶的存在可以解释提取物的不稳定性。
Aspergillus fumigatus grown in submerged and surface cultures was extracted, and the extracts were analyzed separately. The submerged extract contained 31.9% protein and 8.3% carbohydrate, while the corresponding values were 17.0% and 33.3% for the surface material. With individual sera from patients with allergic asthma, SDS‐PAGE combined with immunoblotting revealed that the submerged extract contained at least six strong IgE‐binding components (20, 30, 38, 50, 68, and 90 kDa) in addition to several weak to medium IgE‐binding components. The surface extract contained about the same number of IgE‐binding components, but only one gave a strong reaction (20 kDa). The allergens present were shown to have pI between 4.5 and 5.6 as demonstrated by isoelectric focusing (IEF) combined with immunoblotting. For identification of A. fumigatus glycoprotein allergens, both extracts were treated with periodate under mild conditions. Two allergens of the submerged extract (90 and 38 kDa) partly lost their IgE‐binding ability by this treatment, indicating that these components are glycoproteins and that the carbohydrate moiety is involved in the IgE binding. The IgE‐binding ability of the 20‐kDa allergen was not influenced by periodate. For assessment of the stability of the two allergen extracts, aqueous solutions were kept at 4°C for 2, 7, and 21 d and then analyzed by SDS‐PAGE and immunoblotting. The results showed that most allergens of the submerged extract were partly inactivated after 2 d. After 21 d, only the 20‐kDa and 30‐kDa components were still able to bind IgE. Similar results were obtained by analyzing the surface extract. When the same experiment was performed on samples in a 50% glycerol solution, the results strongly indicated that glycerol had a stabilizing effect on allergens in both extracts. The enzyme content, estimated by the API ZYM‐test, showed that both extracts contained several protein‐ and carbohydrate‐degrading enzymes. The presence of these enzymes may explain the lability of the extracts.
Calvatia cyathiformis 担孢子过敏原的稳定性研究。
DOI：
10.1159/000235020
发表时间：
1989
期刊：
International archives of allergy and applied immunology
影响因子：
--
作者：
Horner,WE;Ibanez,MD;Lehrer,SB
通讯作者：
Lehrer,SB
烟曲霉抗原的酶谱和免疫化学特征。
DOI：
10.1016/0091-6749(86)90267-8
发表时间：
1986
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Kurup,VP;Resnick,A;Scribner,GH;Gunasekaran,M;Fink,JN
通讯作者：
Fink,JN