Small RNA and transcriptional upregulation.
Small RNA and transcriptional upregulation.
复制标题
DOI：
10.1002/wrna.90
复制
发表时间：
2011-09
影响因子：
7.3
通讯作者：
Li, Long-Cheng
中科院分区：
文献类型：
--
作者：
Portnoy, Victoria;Huang, Vera;Place, Robert F.;Li, Long-Cheng
小RNA分子，如microRNA和siRNA，已经成为基因表达的主要调节因子，通过它们在统称为RNA干扰（RNAi）的现象中抑制靶基因的能力。越来越多的证据表明，小RNA也可以通过靶向基因调控序列作为基因表达的激活剂。这种新型机制被称为RNA激活（RNAa），似乎至少在哺乳动物细胞中是保守的，并由内源性和人工设计的小RNA触发。RNAa依赖于Argonaute蛋白，但具有与RNAi不同的动力学。表观遗传学变化与RNA相关，可能有助于靶基因的转录激活，但其潜在机制仍不清楚。鉴于RNA作为研究基因功能的分子工具和作为疾病治疗的潜力，需要进一步的研究来充分阐明其分子机制，以完善靶点选择的规则和改进利用其治疗的策略。
Small RNA molecules, such as microRNA and siRNA, have emerged as master regulators of gene expression through their ability to suppress target genes in a phenomenon collectively called RNA interference (RNAi). There is growing evidence that small RNAs can also serve as activators of gene expression by targeting gene regulatory sequences. This novel mechanism, known as RNA activation (RNAa), appears to be conserved in at least mammalian cells and triggered by both endogenous and artificially designed small RNAs. RNAa depends on Argonaute proteins, but possesses kinetics distinct from that of RNAi. Epigenetic changes are associated with RNAa and may contribute to transcriptional activation of target genes, but the underlying mechanism remains elusive. Given the potential of RNAa as a molecular tool for studying gene function and as a therapeutic for disease, further research is needed to elucidate fully its molecular mechanism in order to refine the rules for target selection and improve strategies for exploiting it therapeutically.
登录
查看更多内容
影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
56.9
作者：
Jopling, CL;Yi, MK;Sarnow, P
通讯作者：
Sarnow, P
影响因子：
4.1
作者：
Aigner, A
通讯作者：
Aigner, A
影响因子：
64.5
作者：
BREITBART, RE;NGUYEN, HT;NADALGINARD, B
通讯作者：
NADALGINARD, B
影响因子：
16.8
作者：
Janowski, Bethany A.;Huffman, Kenneth E.;Corey, David R.
通讯作者：
Corey, David R.