Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomic libraries.
Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomic libraries.
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DOI：
10.1111/j.1462-2920.2009.02049.x
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发表时间：
2010-01
影响因子：
5.1
通讯作者：
Charles TC
中科院分区：
文献类型：
--
作者：
Hao Y;Winans SC;Glick BR;Charles TC
细菌利用群体感应（QS）细胞间通讯系统根据细胞密度协调其行为。目前已鉴定出几种不同类型的QS信号分子，其中对变形菌产生的酰基高丝氨酸内酯（AHL）的研究最多。虽然QS系统在培养微生物中已被广泛研究，但对未培养微生物的QS系统及其在微生物群落中的作用知之甚少。为了扩展我们对QS系统的了解，并更好地理解在自然环境中发生的信号传导，使用来自活性污泥和土壤的DNA构建的宏基因组文库使用农杆菌生物传感器菌株筛选新的QS合酶基因。鉴定了编码QS信号产生的三个cosplay（QS 6 -1、QS 10 -1和QS 10 -2），DNA序列分析显示所有三个克隆编码新的luxI家族阿勒合酶和luxR家族转录调节因子。薄层层析显示，这些LuxI同源蛋白能够合成多种阿勒信号。串联质谱分析显示，LuxIQS 6 -1指导至少三种AHL的合成，即3-O-C14：1 HSL、3-O-C16：1 HSL和3-O-C14 HSL; LuxIQS 10 -1指导至少3-O-C12 HSL和3-O-C14 HSL的合成;而LuxIQS 10 -2指导至少C8 HSL和C10 HSL的合成。两种可能的新AHL：C14：3 HSL和（？）还发现LuxIQS 6 -1合成了羟甲基-3-O-C14 HSL。
Quorum sensing (QS) cell–cell communication systems are utilized by bacteria to coordinate their behaviour according to cell density. Several different types of QS signal molecules have been identified, among which acyl-homoserine lactones (AHLs) produced by Proteobacteria have been studied to the greatest extent. Although QS has been studied extensively in cultured microorganisms, little is known about the QS systems of uncultured microorganisms and the roles of these systems in microbial communities. To extend our knowledge of QS systems and to better understand the signalling that takes place in the natural environment, metagenomic libraries constructed using DNA from activated sludge and soil were screened, using an Agrobacterium biosensor strain, for novel QS synthase genes. Three cosmids (QS6-1, QS10-1 and QS10-2) that encode the production of QS signals were identified and DNA sequence analysis revealed that all three clones encode a novel luxI family AHL synthase and a luxR family transcriptional regulator. Thin layer chromatography revealed that these LuxI homologue proteins are able to synthesize multiple AHL signals. Tandem mass spectrometry analysis revealed that LuxIQS6-1 directs the synthesis of at least three AHLs, 3-O-C14:1 HSL, 3-O-C16:1 HSL and 3-O-C14 HSL; LuxIQS10-1 directs the synthesis of at least 3-O-C12 HSL and 3-O-C14 HSL; while LuxIQS10-2 directs the synthesis of at least C8 HSL and C10 HSL. Two possible new AHLs, C14:3 HSL and (?)-hydroxymethyl-3-O-C14 HSL, were also found to be synthesized by LuxIQS6-1.
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影响因子：
2.8
作者：
Gray, KM;Garey, JR
通讯作者：
Garey, JR
影响因子：
10.7
作者：
Lerat, E;Moran, NA
通讯作者：
Moran, NA
DOI：
10.1073/pnas.84.19.6639
发表时间：
1987-10-01
影响因子：
11.1
作者：
KAPLAN, HB;GREENBERG, EP
通讯作者：
GREENBERG, EP
影响因子：
3.5
作者：
Jansen, GA;Hogenhout, EM;Wanders, RJA
通讯作者：
Wanders, RJA
影响因子：
3.2
作者：
FINAN, TM;KUNKEL, B;SIGNER, ER
通讯作者：
SIGNER, ER