Hollow Fibers for Hepatocyte Encapsulation and Transplantation: Studies of Survival and Function in Rats
Hollow Fibers for Hepatocyte Encapsulation and Transplantation: Studies of Survival and Function in Rats
复制标题
用于肝细胞封装和移植的中空纤维：大鼠存活和功能的研究
作者：
Michael B. Yang;J. Vacanti;D. Ingber
本研究探讨了利用中空纤维（HF）移植肝细胞的可行性。通过体外和体内实验，研究了四种不同类型的市售HF：再生纤维素HF （RCHF）、两种不同尺寸的聚砜HF （PSHF-1和PSHF-2）和聚乙烯基HF （PVDF）包封的肝细胞的活力和功能。通过光镜检测和肝细胞合成蛋白质和分泌白蛋白的能力，所有HF类型的肝细胞在体外存活至少1周。然而，这些细胞的蛋白质合成和白蛋白分泌水平在不同HF （RCHF > PSHF-2 > PVDF ã PSHF-1）之间存在显著差异，并且似乎与它们的内径（RCHF、PSHF-2、PVDF和PSHF-1分别为215、500、1000和1100 μm）呈负相关。虽然PSHF-2、PVDF和PSHF-1在体内不支持长期生存，但RCHF中的肝细胞在植入肠系膜后存活。在体内培养24小时后，肝细胞形态完整，与平行培养的细胞相比，蛋白质合成率相似。RCHF组的肝细胞在体内7天后也保持了合成蛋白质的能力。这些结果表明，适当大小的HF可能对无法实现预血管化的肝细胞移植应用有用。
In this study, the feasibility of transplanting hepatocytes using hollow fibers (HF) was investigated. Experiments were carried out in vitro and in vivo to determine the viability and function of hepatocytes encapsulated in four different types of commercially available HF: regenerated cellulose HF (RCHF), polysulfone HF of two different sizes (PSHF-1 and PSHF-2), and polyvinylidine HF (PVDF). Hepatocytes remained viable in all types of HF for at least 1 wk in vitro as measured by light microscopy and their ability to synthesize protein and secrete albumin. However, the levels of protein synthesis and albumin secretion in these cells varied significantly between different HF (RCHF > PSHF-2 > PVDF ã PSHF-1) and appeared to be inversely related to their internal diameters (215, 500, 1000, and 1100 μm for RCHF, PSHF-2, PVDF, and PSHF-1, respectively). While PSHF-2, PVDF, and PSHF-1 did not support long term viability in vivo, hepatocytes in RCHF survived after implantation in the mesentery. After 24 h in vivo, the hepatocytes appeared morphologically intact and exhibited a similar rate of protein synthesis when compared with cells cultured in parallel. The hepatocytes in RCHF also maintained the ability to synthesize protein after 7 days in vivo. These results suggest that HF of appropriate size may be useful for hepatocyte transplantation applications in which prevascularization is not possible.
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DOI：
10.1073/pnas.88.4.1217
发表时间：
1991-02-01
影响因子：
11.1
作者：
PONDER, KP;GUPTA, S;WOO, SLC
通讯作者：
WOO, SLC
DOI：
--
发表时间：
1986
期刊：
The American journal of pathology
影响因子：
--
作者：
D. Ingber;J. Madri;J. Jamieson
通讯作者：
D. Ingber;J. Madri;J. Jamieson
影响因子：
56.9
作者：
Jan Thompson;Anderson Kd;JM DiPietro;J. Zwiebel;M. Zametta;W. Anderson;T. Maciag
通讯作者：
Jan Thompson;Anderson Kd;JM DiPietro;J. Zwiebel;M. Zametta;W. Anderson;T. Maciag
生物人工肝的发育：Gunn 大鼠中的胆红素结合。
DOI：
10.1016/0022-4804(90)90079-h
发表时间：
1990
期刊：
The Journal of surgical research
影响因子：
--
作者：
Arnaout,WS;Moscioni,AD;Barbour,RL;Demetriou,AA
通讯作者：
Demetriou,AA
DOI：
10.1073/pnas.85.7.2161
发表时间：
1988-04-01
影响因子：
11.1
作者：
BENZEEV, A;ROBINSON, GS;FARMER, SR
通讯作者：
FARMER, SR