Writing DNA with GenoCAD.

Writing DNA with GenoCAD.
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DOI:
10.1093/nar/gkp361
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发表时间:
2009-07
影响因子:
14.9
通讯作者:
Peccoud J
Peccoud J
中科院分区:
生物学2区
文献类型:
--
作者:
Czar MJ;Cai Y;Peccoud J

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定制DNA的化学合成需要软件来简化合成DNA序列的设计。GenoCAD™(www.genocad.org)是一个免费的基于网络的应用程序,用于设计蛋白质表达载体、人工基因网络和其他由称为遗传部分的多个功能块组成的遗传构建体。通过捕获DNA序列语法模型中的设计策略,GenoCAD引导用户完成设计过程。通过连续点击代表结构特征或实际遗传部分的图标,可以在几分钟内设计出由数十个功能块组成的复杂结构。GenoCAD自动从其全面的遗传部件库中推导出构建序列。设计过程完成后,用户可以下载序列进行合成或进一步分析。选择在系统上创建个人帐户的用户可以通过创建自己的零件库、向零件库添加新零件或重用设计来快速生成相关构件集,从而自定义其工作空间。
Chemical synthesis of custom DNA made to order calls for software streamlining the design of synthetic DNA sequences. GenoCAD™ (www.genocad.org) is a free web-based application to design protein expression vectors, artificial gene networks and other genetic constructs composed of multiple functional blocks called genetic parts. By capturing design strategies in grammatical models of DNA sequences, GenoCAD guides the user through the design process. By successively clicking on icons representing structural features or actual genetic parts, complex constructs composed of dozens of functional blocks can be designed in a matter of minutes. GenoCAD automatically derives the construct sequence from its comprehensive libraries of genetic parts. Upon completion of the design process, users can download the sequence for synthesis or further analysis. Users who elect to create a personal account on the system can customize their workspace by creating their own parts libraries, adding new parts to the libraries, or reusing designs to quickly generate sets of related constructs.
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