α Smooth muscle actin positive stromal cells in gastric carcinoma
α Smooth muscle actin positive stromal cells in gastric carcinoma
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胃癌中α平滑肌肌动蛋白阳性基质细胞
DOI：
10.1136/jcp.55.10.741
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发表时间：
2002
影响因子：
3.4
通讯作者：
M. Toi
中科院分区：
文献类型：
--
作者：
H. Nakayama;H. Enzan;E. Miyazaki;M. Toi
目的：探讨肌成纤维细胞群中α平滑肌肌动蛋白（ASMA）阳性基质细胞（ASMA+细胞）在弥漫型、肠型和实体型胃癌中的分布及作用。方法：74例手术切除的胃癌（弥漫型24例，肠型43例，实体型7例）。ASMA阳性和高分子量钙调蛋白（HCD）阴性的基质细胞被视为ASMA+细胞。免疫组化法分析CD 34阳性基质细胞（CD 34+细胞）的分布。结果如下：在24例弥漫型胃癌中，13例浸润浆膜下的癌中有6例在肿瘤间质中有ASMA+细胞，而所有6例局限于粘膜下层的弥漫型胃癌和所有5例浸润固有肌层的胃癌在肿瘤间质中没有ASMA+细胞。在43例胃粘膜型胃癌中，仅5/21例局限于粘膜下层的癌在肿瘤间质中有ASMA+细胞，而21/22例侵犯固有肌层和浆膜下的胃粘膜型胃癌在肿瘤间质中有ASMA+细胞束。CD 34+细胞在弥漫型和弥漫型胃癌中的分布与以前发表的系列研究中所见相似。所有七个实体型胃癌检查有ASMA+细胞，但没有CD 34+细胞在肿瘤间质。在弥漫型肿瘤中未检测到ASMA和CD 34双阳性的基质细胞。结论：这些结果表明，ASMA在间质细胞中的表达与浸润浆膜下的弥漫型胃癌、浸润固有肌层和浆膜下的胃粘膜型胃癌以及实体型胃癌的肿瘤间质形成相关。
Aims: To investigate the distribution and roles of α smooth muscle actin (ASMA) positive stromal cells (ASMA+ cells), which belong to the myofibroblast group, within gastric carcinomas, with reference to three histological types (diffuse type, intestinal type, and solid type). Methods: In total, 74 surgically resected gastric carcinomas (24 diffuse type, 43 intestinal type, and seven solid type) were examined. ASMA positive and high molecular weight caldesmon (HCD) negative stromal cells were regarded as ASMA+ cells. The distribution of CD34 positive stromal cells (CD34+ cells) was also analysed immunohistochemically. Results: In the 24 diffuse-type gastric carcinomas, six of the 13 carcinomas invading the subserosa had ASMA+ cells in the tumour stroma, whereas all six diffuse-type gastric carcinomas confined to the submucosa and all five invading the muscularis propria had no ASMA+ cells in the tumour stroma. In the 43 intestinal-type gastric carcinomas, only five of the 21 carcinomas confined to the submucosa had ASMA+ cells in the tumour stroma, whereas 21 of the 22 intestinal-type gastric carcinomas invading the muscularis propria and the subserosa had ASMA+ cell bundles in the tumour stroma. The distribution of CD34+ cells in diffuse-type and intestinal-type gastric carcinomas was similar to that seen in a previously published series. All seven solid-type gastric carcinomas examined had ASMA+ cells but not CD34+ cells in the tumour stroma. No stromal cells double positive for ASMA and CD34 were detected within the diffuse-type tumours examined. Conclusions: These results suggest that ASMA expression in stromal cells is associated with tumour stroma formation of diffuse-type gastric carcinomas invading the subserosa, intestinal-type gastric carcinomas invading the muscularis propria and subserosa, and solid-type gastric carcinomas.
DOI：
10.1073/pnas.90.3.999
发表时间：
1993-02-01
影响因子：
11.1
作者：
LAZARD, D;SASTRE, X;KOTELIANSKY, VE
通讯作者：
KOTELIANSKY, VE