Functioning nanomachines seen in real-time in living bacteria using single-molecule and super-resolution fluorescence imaging.
Functioning nanomachines seen in real-time in living bacteria using single-molecule and super-resolution fluorescence imaging.
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DOI：
10.3390/ijms12042518
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发表时间：
2011
影响因子：
5.6
通讯作者：
Leake MC
中科院分区：
文献类型：
--
作者：
Chiu SW;Leake MC
分子机器是“预先建立的”纳米技术的例子，驱动着活细胞的基本生物化学。它们涵盖了广泛的功能，包括化学过程的燃料生成，细胞内分子组分的运输，细胞流动性，信号转导和遗传密码的复制等。我们对这种纳米尺度机器的大部分理解都来自于在隔离的人工条件下进行的体外研究。研究人员现在正在应对在其原生环境中研究纳米机器的挑战。在这篇综述中，我们概述了最近在模型细菌系统中使用最先进的遗传学技术结合尖端的单分子和超分辨率荧光显微镜的纳米机器的体内调查。我们的结论是，单分子和超分辨率荧光成像生物纳米机器的生化，结构和功能表征提供了强大的工具。从荧光成像中同时获得的整合的空间、时间和单分子数据为系统水平的单分子细胞生物物理学和体内生物化学开辟了一条途径。
Molecular machines are examples of “pre-established” nanotechnology, driving the basic biochemistry of living cells. They encompass an enormous range of function, including fuel generation for chemical processes, transport of molecular components within the cell, cellular mobility, signal transduction and the replication of the genetic code, amongst many others. Much of our understanding of such nanometer length scale machines has come from in vitro studies performed in isolated, artificial conditions. Researchers are now tackling the challenges of studying nanomachines in their native environments. In this review, we outline recent in vivo investigations on nanomachines in model bacterial systems using state-of-the-art genetics technology combined with cutting-edge single-molecule and super-resolution fluorescence microscopy. We conclude that single-molecule and super-resolution fluorescence imaging provide powerful tools for the biochemical, structural and functional characterization of biological nanomachines. The integrative spatial, temporal, and single-molecule data obtained simultaneously from fluorescence imaging open an avenue for systems-level single-molecule cellular biophysics and in vivo biochemistry.
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影响因子：
48
作者：
Biteen, Julie S.;Thompson, Michael A.;Tselentis, Nicole K.;Bowman, Grant R.;Shapiro, Lucy;Moerner, W. E.
通讯作者：
Moerner, W. E.
DOI：
10.1073/pnas.0401638101
发表时间：
2004-04-27
影响因子：
11.1
作者：
Gordon, MP;Ha, T;Selvin, PR
通讯作者：
Selvin, PR
影响因子：
3.2
作者：
Brown, Perry N.;Terrazas, Moises;Blair, David F.
通讯作者：
Blair, David F.
影响因子：
3.4
作者：
Balci, H;Ha, T;Selvin, PR
通讯作者：
Selvin, PR
影响因子：
7.2
作者：
Biteen, Julie S.;Moerner, W. E.
通讯作者：
Moerner, W. E.