Some aspects of the iodine metabolism of the giant kelp Macrocystis pyrifera (phaeophyceae).
Some aspects of the iodine metabolism of the giant kelp Macrocystis pyrifera (phaeophyceae).
复制标题
巨藻Macrocystis Pyrifera（褐藻纲）碘代谢的某些方面。
DOI：
10.1016/j.jinorgbio.2017.09.003
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发表时间：
2017
影响因子：
3.9
通讯作者：
C. Carrano
中科院分区：
文献类型：
--
作者：
Teresa Tymon;E. P. Miller;Jennifer Gonzales;A. Raab;F. Küpper;C. Carrano
尽管巨藻在沿海生态系统的功能中起着至关重要的作用，但人们对巨藻（Macrocystispyrifera）的卤素代谢知之甚少。考虑到大囊藻广泛分布范围内的海洋和大气化学过程的潜在影响，这是一个重要的缺点。这里提出的工作构成了第一次深入研究碘的摄取，外排和生理功能在这个重要的海带物种。在正常和应激（外源性过氧化氢和激发剂触发的氧化爆发）条件下，测量了大囊藻成年孢子体的碘吸收和外排率。海带组织在含不同浓度碘的海水中孵育时，根据Michaelis-Menten型动力学吸收碘。在加入外源性过氧化氢后，模拟氧化应激，发生了明显的碘化物外流。在大囊藻中，加入海藻酸酯的低聚降解产物以及花生四烯酸和茉莉酸甲酯，引发了过氧化氢的原位生成，形成了可能与碘积累有关的防御性氧化爆发。在单细胞水平上用二氯氢荧光素检测H2O2，二氯氢荧光素是一种能够检测细胞内H2O2的荧光探针。当测定钒卤素过氧化物酶活性时，检测到几种溴过氧化物酶异构体以及一种碘过氧化物酶。总之，本研究的结果表明，大囊藻具有复杂的碘代谢，这可能对影响海带床周围海水中的碘物种形成和沿海大气中挥发性卤素的排放具有重要意义。
Despite its paramount role in the functioning of coastal ecosystems, relatively little is known about halogen metabolism in giant kelp (Macrocystispyrifera). This is an important shortcoming given the potential implications for marine and atmospheric chemical processes in the wide distribution range ofMacrocystis. The work presented here constitutes the first in depth investigation of the uptake, efflux, and of the physiological function of iodide in this important kelp species. Iodide uptake and efflux rates were measured in adult sporophytes ofMacrocystisunder normal and stressed (exogenous hydrogen peroxide and an elicitor-triggered oxidative burst) conditions. Kelp tissue took up iodide according to Michaelis-Menten type kinetics when incubated in seawater enriched with various concentrations of iodide. Upon the addition of exogenous hydrogen peroxide, simulating oxidative stress, a marked efflux of iodide occurred. In situ generation of hydrogen peroxide was elicited inMacrocystisupon the addition of oligomeric degradation products of alginate as well as arachidonic acid and methyl jasmonate constituting a defensive oxidative burst that could be linked to iodine accumulation. H2O2was detected at the single cell level using dichlorohydrofluorescein diacetate, a fluorogenic probe capable of detecting intracellular H2O2. When assayed for vanadium haloperoxidase activity, several bromoperoxidase isoforms were detected as well as a single iodoperoxidase. Altogether, the results of this study show thatMacrocystishas an elaborate iodine metabolism, which is likely significant for impacting iodine speciation in seawater around kelp beds and for volatile halogen emissions into the coastal atmosphere.
影响因子：
64.8
作者：
Cock, J. Mark;Sterck, Lieven;Wincker, Patrick
通讯作者：
Wincker, Patrick