Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level
Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level
复制标题
幽门螺杆菌基因组图谱的构建和基因组水平多样性的展示
作者：
Diane E. Taylor;M. Eaton;N. Chang;S. Salama
来自 30 株幽门螺杆菌的基因组 DNA 在用 NotI 和 NruI 消化后进行脉冲场凝胶电泳 (PFGE)。菌株的基因组大小范围为 1.6 至 1.73 Mb，平均大小为 1.67 Mb。通过使用NotI和NruI，构建了含有三个拷贝的16S和23S rRNA基因的幽门螺杆菌UA802（1.7 Mb）的环形图谱。幽门螺杆菌基因组的一个不寻常特征是至少两个 16S 和 23S rRNA 基因拷贝的独立位置。几乎所有菌株在 NotI 和 NruI 消化后都有不同的 PFGE 模式，表明幽门螺杆菌基因组具有相当程度的遗传变异性。然而，同一患者体内不同部位（胃底、胃窦和胃体）的三种菌株给出了相同的 PFGE 模式。单个分离株的基因组模式在体外多次传代培养期间保持恒定。在幽门螺杆菌菌株中观察到的遗传多样性的原因仍有待解释。
Genomic DNA from 30 strains of Helicobacter pylori was subjected to pulsed-field gel electrophoresis (PFGE) after digestion with NotI and NruI. The genome sizes of the strains ranged from 1.6 to 1.73 Mb, with an average size of 1.67 Mb. By using NotI and NruI, a circular map of H. pylori UA802 (1.7 Mb) which contained three copies of 16S and 23S rRNA genes was constructed. An unusual feature of the H. pylori genome was the separate location of at least two copies of 16S and 23S rRNA genes. Almost all strains had different PFGE patterns after NotI and NruI digestion, suggesting that the H. pylori genome possesses a considerable degree of genetic variability. However, three strains from different sites (the fundus, antrum, and body of the stomach) within the same patient gave identical PFGE patterns. The genomic pattern of individual isolates remained constant during multiple subcultures in vitro. The reason for the genetic diversity observed among H. pylori strains remains to be explained.
大肠杆菌 K12 基因组的物理图谱。
DOI：
10.1126/science.3296194
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Smith,CL;Econome,JG;Schutt,A;Klco,S;Cantor,CR
通讯作者：
Cantor,CR
影响因子：
158.5
作者：
PARSONNET, J;FRIEDMAN, GD;SIBLEY, RK
通讯作者：
SIBLEY, RK
影响因子：
158.5
作者：
NOMURA, A;STEMMERMANN, GN;BLASER, MJ
通讯作者：
BLASER, MJ