Enhanced fluorescence images for labeled cells on silver island films.
Enhanced fluorescence images for labeled cells on silver island films.
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DOI：
10.1021/la801749f
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发表时间：
2008-11-04
期刊：
影响因子：
3.9
通讯作者：
Lakowicz, Joseph R.
中科院分区：
文献类型：
--
作者：
Zhang, Jian;Fu, Yi;Liang, Dong;Zhao, Richard Y.;Lakowicz, Joseph R.
银岛膜（SIF）沉积在玻璃基板上作为支撑。 T 淋巴细胞 (PM1) 细胞系分别用膜上的 Alexa Fluor 680 葡聚糖缀合物或细胞核中的 YOYO 进行标记。使用扫描共焦显微镜记录细胞系的荧光图像的发射强度和寿命。与玻璃上相比，SIF 支持物显着增强了结合在细胞膜上的荧光团的荧光信号。除了强度增加之外，发射寿命也急剧缩短。与膜上的 Alexa Fluor 680 荧光团相比，嵌入细胞核中的 YOYO 荧光团并未受到银岛的显着影响。该结果可以通过距离对荧光团和金属颗粒之间的耦合的影响来解释：细胞膜上的荧光团位于金属增强荧光区域内，但细胞核中的荧光团超出金属增强荧光区域。因此，当细胞表面的目标分子被荧光标记时，金属支持物可用于提高对它们的检测灵敏度。
Silver island films (SIFs) were deposited on the glass substrates to serve as the supports. T-lymphocytic (PM1) cell lines were labeled by Alexa Fluor 680 dextran conjugates on the membranes or by YOYO in the nucleus, respectively. The fluorescence images of the cell lines were recorded in the emission intensity and lifetime using scanning confocal microscopy. The fluorescence signals by the fluorophores bound on the cell membranes were enhanced significantly by SIF supports as compared with on the glass. In addition to the increase in the intensity, there was a dramatic shortening of the emission lifetime. In contrast to the Alexa Fluor 680 fluorophores on the membranes, the YOYO fluorophores intercalated in the cell nucleus were not influenced significantly by the silver islands. This result can be interpreted by an effect of the distance on coupling between the fluorophores and metal particles: the fluorophores on the cell membranes are localized within but the fluorophores in the cell nucleus are beyond the region of metal-enhanced fluorescence. Thus, the metal supports can be used to improve the detection sensitivity to the target molecules on the cell surfaces when they are fluorescently labeled.
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