Production of Sry knockout mouse using TALEN via oocyte injection.
Production of Sry knockout mouse using TALEN via oocyte injection.
复制标题
DOI：
10.1038/srep03136
复制
发表时间：
2013-11-05
影响因子：
4.6
通讯作者：
Takada, Shuji
中科院分区：
文献类型：
--
作者：
Kato, Tomoko;Miyata, Kohei;Sonobe, Miku;Yamashita, Satoshi;Tamano, Moe;Miura, Kento;Kanai, Yoshiakira;Miyamoto, Shingo;Sakuma, Tetsushi;Yamamoto, Takashi;Inui, Masafumi;Kikusui, Takefumi;Asahara, Hiroshi;Takada, Shuji
最近开发的转录激活因子样效应核酸酶（TALEN）技术已经能够创建敲除小鼠，即使是Y染色体上的基因。在这项研究中，我们产生了一个敲除小鼠Sry，Y染色体上的性别决定基因，使用显微注射TALEN RNA到原核期卵母细胞。正如预期的那样，基因敲除小鼠具有雌性的外生殖器和内生殖器，血液睾酮水平为雌性，大脑中有一个雌性性二型核。基因敲除小鼠表现出发情周期，并进行交配行为的女性，虽然它是不育或生育能力降低。组织学分析显示，敲除小鼠的卵巢显示卵母细胞数量减少和黄素化未破裂卵泡，这意味着排卵卵母细胞数量减少是KO小鼠不育和/或生育力降低的可能原因。
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the Y chromosome, using microinjection of TALEN RNA into pronuclear stage oocytes. As expected, the knockout mouse had female external and internal genitalia, a female level of blood testosterone and a female sexually dimorphic nucleus in the brain. The knockout mouse exhibited an estrous cycle and performed copulatory behavior as females, although it was infertile or had reduced fertility. A histological analysis showed that the ovary of the knockout mouse displayed a reduced number of oocytes and luteinized unruptured follicles, implying that a reduced number of ovulated oocytes is a possible reason for infertility and/or reduced fertility in the KO mouse.
登录
查看更多内容
影响因子：
4.6
作者：
Mashimo, Tomoji;Kaneko, Takehito;Sakuma, Tetsushi;Kobayashi, Junya;Kunihiro, Yayoi;Voigt, Birger;Yamamoto, Takashi;Serikawa, Tadao
通讯作者：
Serikawa, Tadao
DOI：
10.1073/pnas.1137864100
发表时间：
2003-06-10
影响因子：
11.1
作者：
Harley, VR;Layfield, S;Jans, DA
通讯作者：
Jans, DA
影响因子：
14.9
作者：
Cade L;Reyon D;Hwang WY;Tsai SQ;Patel S;Khayter C;Joung JK;Sander JD;Peterson RT;Yeh JR
通讯作者：
Yeh JR
影响因子：
56.9
作者：
Boch, Jens;Scholze, Heidi;Bonas, Ulla
通讯作者：
Bonas, Ulla
影响因子：
3.2
作者：
Anderson, GM;Connors, JM;Goodman, RL
通讯作者：
Goodman, RL