Two non-target recessive genes confer resistance to the anti-oomycete microtubule inhibitor zoxamide in Phytophthora capsici.
Two non-target recessive genes confer resistance to the anti-oomycete microtubule inhibitor zoxamide in Phytophthora capsici.
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DOI：
10.1371/journal.pone.0089336
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Bi Y;Chen L;Cai M;Zhu S;Pang Z;Liu X
这项研究对辣椒疫霉分离株进行了鉴定，这些辣椒疫霉分离株已开发出一种新的苯佐沙胺抗性机制，该机制改变了最低抑制浓度 (MIC)，但不改变 EC50。分子分析显示，耐药菌株的β-微管蛋白基因不包含突变，并且表达水平与苯佐沙胺敏感菌株相同。这表明辣椒疫霉已经对苯佐沙胺产生了一种新型的非靶点抗性。对敏感菌株PCAS1和PCAS2的有性后代中苯佐沙胺抗性分离比的分析表明，对苯佐沙胺的抗性是由一个或多个隐性核基因控制的。此外，F1、F2和BC1后代的抗性分离与χ2检验的理论比值一致（P>0.05），这表明对苯佐沙胺的抗性是由两个隐性基因控制的，并且当这些等位基因中至少有一对纯合时，就会发生对苯佐沙胺的抗性。这意味着辣椒疫病菌对苯佐沙胺产生抗性的风险为低至中等。然而，应该密切监测这种潜在的抵抗力，特别是如果两种兼容的交配类型在同一领域中共存。
This study characterized isolates of P. capsici that had developed a novel mechanism of resistance to zoxamide, which altered the minimum inhibition concentration (MIC) but not the EC50. Molecular analysis revealed that the β-tubulin gene of the resistant isolates contained no mutations and was expressed at the same level as in zoxamide-sensitive isolates. This suggested that P. capsici had developed a novel non-target-site-based resistance to zoxamide. Analysis of the segregation ratio of zoxamide-resistance in the sexual progeny of the sensitive isolates PCAS1 and PCAS2 indicated that the resistance to zoxamide was controlled by one or more recessive nuclear genes. Furthermore, the segregation of resistance in the F1, F2, and BC1 progeny was in accordance with the theoretical ratios of the χ2 test (P>0.05), which suggested that the resistance to zoxamide was controlled by two recessive genes, and that resistance to zoxamide occurred when at least one pair of these alleles was homozygous. This implies that the risk of zoxamide-resistance in P. capsici is low to moderate. Nevertheless this potential for resistance should be monitored closely, especially if two compatible mating types co-exist in the same field.
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影响因子：
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