Abdominal Massage Improves the Symptoms of Irritable Bowel Syndrome by Regulating Mast Cells via the Trypase-PAR2-PKCε Pathway in Rats.
Abdominal Massage Improves the Symptoms of Irritable Bowel Syndrome by Regulating Mast Cells via the Trypase-PAR2-PKCε Pathway in Rats.
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腹部按摩通过胰蛋白酶-PAR2-PKCγ途径调节肥大细胞改善大鼠肠易激综合征的症状
DOI：
10.1155/2022/8331439
复制
发表时间：
2022
影响因子：
2.9
通讯作者：
Wang, Jingui
中科院分区：
文献类型：
--
作者：
Li, Huanan;Zhang, Wei;Ma, Fei;Zhang, Xiaofan;Wang, Yuyan;Wang, Jingui
肠易激综合征（IBS）是一种临床疾病，主要表现为腹痛和不适综合征，常见于20 - 50岁的人群。腹部按摩对人体健康具有重要的医学意义，包括促进肠道蠕动、缓解便秘以及有助于减肥。然而，其在缓解IBS方面的潜在益处及其潜在机制仍不明确。
在本研究中，我们建立了大鼠IBS模型以评估腹部按摩的效果。40只雄性斯普拉格 - 道利（SD）大鼠随机分为4组：正常（对照）组、IBS组、腹部按摩组以及腹部按摩 + 酮替芬治疗组（每组n = 10只大鼠）。腹部按摩每天进行一次，每次5分钟，持续14天。在第14天，对大鼠实施安乐死，并通过透射电子显微镜（TEM）、免疫组织化学或免疫荧光染色以及激光共聚焦聚焦来分析组织，以观察肠黏膜的微观形态。通过实时定量聚合酶链反应（RT - qPCR）和蛋白质印迹法（western blot）测定瞬时受体电位香草酸亚型1（TRPV1）的表达以及类胰蛋白酶的释放。
我们发现，与对照组相比，IBS组的肥大细胞显著增加，而经过腹部按摩（无论是否使用酮替芬治疗），增加的肥大细胞数量部分减少。我们还发现IBS组中TRPV1表达上调。腹部按摩（无论是否使用酮替芬治疗）能够减弱IBS中TRPV1的上调。从机制上讲，免疫组织化学和蛋白质印迹法的结果表明，腹部按摩通过调节类胰蛋白酶 - PAR2 - PKCε通路降低了IBS的敏感性。
总体而言，我们的研究结果表明，腹部按摩通过减少肥大细胞募集以及减弱类胰蛋白酶 - PAR2 - PKCε通路，对改善IBS症状产生有益效果。酮替芬能够促进腹部按摩对IBS治疗的效果，这可作为IBS的一种潜在治疗策略。
Irritable bowel syndrome (IBS) is a clinical disease mainly characterized as a syndrome of abdominal pain and discomfort, which frequently occurs in humans aged 20–50. Abdomen massage is of great medical significance for the health of the human body, including promoting intestinal peristalsis, relieving constipation, and facilitating weight loss. However, its potential benefits in alleviating IBS and the underlying mechanisms remain elusive. In this study, we established an IBS model in rats to evaluate the effects of abdomen massage. Forty male Sprague Dawley (SD) rats were randomly assigned into 4 groups: the normal (control) group, IBS group, abdominal massage group, and abdominal massage + ketotifen treatment group (n = 10 rats in each group). Abdominal massage was performed once a day for 5 minutes for 14 days. On day 14, the rats were euthanized and the tissues were analyzed by transmission electron microscopy (TEM), immunohistochemistry or immunofluorescence staining, and laser confocal focus to visualize the micromorphology of the intestinal mucosa. The expression of TRPV1 and the release of trypase were determined by RT-qPCR and western blot. We found that compared with the control group, the mast cells in the IBS group were significantly increased and the increased MC was partially decreased by an abdominal massage with or without ketotifen treatment. We also found that TRPV1 was upregulated in the IBS group. Abdominal massage with or without ketotifen treatment could attenuate the upregulation of TRPV1 in IBS. Mechanically, results of IHC and western Blot suggested that abdominal massage reduces the sensitivity of IBS by regulating the trypase-PAR2-PKCε pathway. Overall, our results suggested that abdominal massage produces a beneficial effect in improving the symptoms of IBS through reducing mast cell recruitment and attenuating the trypase-PAR2-PKCε pathway. Ketotifen could promote the effect of abdominal massage on IBS treatment, which can serve as a potential therapeutic strategy for IBS.
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DOI：
10.1155/2014/218383
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
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作者：
Liu Y;Liu W;Peng QX;Peng JL;Yu LZ;Hu JL
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DOI：
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2021-02-08
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Sensors (Basel, Switzerland)
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2017
期刊：
Evidence-based complementary and alternative medicine : eCAM
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DOI：
10.1016/bs.apha.2016.06.002
发表时间：
2017
期刊：
Advances in pharmacology (San Diego, Calif.)
影响因子：
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