Quantitative trait loci analysis of glucosinolate, sugar, and organic acid concentrations in Eruca vesicaria subsp. sativa.
Quantitative trait loci analysis of glucosinolate, sugar, and organic acid concentrations in Eruca vesicaria subsp. sativa.
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DOI：
10.1186/s43897-022-00044-x
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发表时间：
2022-10-10
影响因子：
--
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中科院分区：
文献类型：
--
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n.囊叶芸苔紫苜蓿是一种以辛辣著称的菊科多叶蔬菜。生长条件、叶龄、农艺措施和品种选择的变化导致质量不一致，特别是异硫氰酸酯（ITC）及其前体芥子油苷（GSL）的含量。我们提出了第一个连锁和数量性状基因座（QTL）图谱芸苔属，使用139个F4系的人口。一个显着的环境影响的丰富的初级和次级代谢产物进行了观察，与英国种植的植物含有显着较高浓度的葡萄糖苷，苹果酸，和总糖。意大利种植的植物的特点是高浓度的葡萄糖，吲哚GSL，和低单糖。20个QTL被鉴定并与稳健的SNP标记相关联。与GSL 4-甲氧基葡萄糖巴西菌素（4 MGB）的合成相关的5个基因pupletin被鉴定为潜在QTL的候选调节因子。分析显示，LG 1上存在的MYB 51、IGMT 1和IGMT 4的直系同源物与芸苔属中的4 MGB浓度相关。这项研究说明了地图的实用性，用于确定与营养成分相关的基因在芸苔属和其价值作为遗传资源，以协助育种计划，这种叶菜作物。在线版本包含补充材料，可通过10.1186/s43897-022-00044-x获得。
Eruca vesicaria subsp. sativa is a leafy vegetable of the Brassicaceae family known for its pungency. Variation in growing conditions, leaf age, agronomic practices, and variety choice lead to inconsistent quality, especially in content of isothiocyanates (ITCs) and their precursor glucosinolates (GSLs). We present the first linkage and Quantitative Trait Loci (QTL) map for Eruca, generated using a population of 139 F4 lines. A significant environmental effect on the abundance of primary and secondary metabolites was observed, with UK-grown plants containing significantly higher concentrations of glucoraphanin, malic acid, and total sugars. Italian-grown plants were characterized by higher concentrations of glucoerucin, indolic GSLs, and low monosaccharides. 20 QTL were identified and associated with robust SNP markers. Five genes putatively associated with the synthesis of the GSL 4-methoxyglucobrassicin (4MGB) were identified as candidate regulators underlying QTL. Analysis revealed that orthologs of MYB51, IGMT1 and IGMT4 present on LG1 are associated with 4MGB concentrations in Eruca. This research illustrates the utility of the map for identifying genes associated with nutritional composition in Eruca and its value as a genetic resource to assist breeding programs for this leafy vegetable crop. The online version contains supplementary material available at 10.1186/s43897-022-00044-x.
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影响因子：
5.6
作者：
Millard PS;Kragelund BB;Burow M
通讯作者：
Burow M
DOI：
10.3390/foods10051055
发表时间：
2021-05-11
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Bell L;Kitsopanou E;Oloyede OO;Lignou S
通讯作者：
Lignou S
影响因子：
5.6
作者：
Bell L;Chadwick M;Puranik M;Tudor R;Methven L;Kennedy S;Wagstaff C
通讯作者：
Wagstaff C
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
影响因子：
7.2
作者：
Gigolashvili, Tamara;Berger, Bettina;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo