The Eruca sativa Genome and Transcriptome: A Targeted Analysis of Sulfur Metabolism and Glucosinolate Biosynthesis Pre and Postharvest.
The Eruca sativa Genome and Transcriptome: A Targeted Analysis of Sulfur Metabolism and Glucosinolate Biosynthesis Pre and Postharvest.
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DOI：
10.3389/fpls.2020.525102
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发表时间：
2020
影响因子：
5.6
通讯作者：
Wagstaff C
中科院分区：
文献类型：
--
作者：
Bell L;Chadwick M;Puranik M;Tudor R;Methven L;Kennedy S;Wagstaff C
火箭(Eruca Sativa)是一种与健康相关的代谢物，称为硫代葡萄糖酸盐(GSLS)和异硫氰酸酯(ITCs)，但对负责调节采前和采后积累的遗传和转录机制知之甚少。我们介绍了第一个从头开始的参考基因组组装和注释，以及与硫的同化、运输和利用有关的个体和收获后转录组数据。火箭自交系之间存在着与硫代谢、GSL生物合成和谷胱甘肽生物合成相关的不同基因表达谱。明确的差异表达模式决定了GSL的丰度和水解物的形成。一个育种系在整个贮藏过程中维持GSL的积累和水解物的形成。MYB28、SLIM1、SDI1和ESM1的多个拷贝在采后增加和差异表达，并与GSLS和水解物的形成有关。两个硫代葡萄糖苷转运蛋白基因(GTR2)拷贝被发现与叶片中GSL积累增加有关。叶片中的单糖(对初级代谢和GSL生物合成至关重要，并对火箭的味道有贡献)也被定量，其中葡萄糖浓度与许多GSL相关基因的表达显著相关。谷胱甘肽合成酶(GSH)基因的表达与GSL代谢相关基因的表达呈显著负相关。与其他两个品系相反，育种品系“B”表现出较高的GSH基因表达和较低的GSL含量。共表达分析表明，衰老基因(SEN1)和氧化胁迫相关基因(OXS3)在品系B中有较高的表达，表明采后品质的恶化与低浓度的GSL有关。
Rocket (Eruca sativa) is a source of health-related metabolites called glucosinolates (GSLs) and isothiocyanates (ITCs) but little is known of the genetic and transcriptomic mechanisms responsible for regulating pre and postharvest accumulations. We present the first de novo reference genome assembly and annotation, with ontogenic and postharvest transcriptome data relating to sulfur assimilation, transport, and utilization. Diverse gene expression patterns related to sulfur metabolism, GSL biosynthesis, and glutathione biosynthesis are present between inbred lines of rocket. A clear pattern of differential expression determines GSL abundance and the formation of hydrolysis products. One breeding line sustained GSL accumulation and hydrolysis product formation throughout storage. Multiple copies of MYB28, SLIM1, SDI1, and ESM1 have increased and differential expression postharvest, and are associated with GSLs and hydrolysis product formation. Two glucosinolate transporter gene (GTR2) copies were found to be associated with increased GSL accumulations in leaves. Monosaccharides (which are essential for primary metabolism and GSL biosynthesis, and contribute to the taste of rocket) were also quantified in leaves, with glucose concentrations significantly correlated with the expression of numerous GSL-related genes. Significant negative correlations were observed between the expression of glutathione synthetase (GSH) genes and those involved in GSL metabolism. Breeding line “B” showed increased GSH gene expression and low GSL content compared to two other lines where the opposite was observed. Co-expression analysis revealed senescence (SEN1) and oxidative stress-related (OXS3) genes have higher expression in line B, suggesting that postharvest deterioration is associated with low GSL concentrations.
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影响因子：
3.4
作者：
Arias, Tatiana;Pires, J. Chris
通讯作者：
Pires, J. Chris
影响因子：
7.2
作者：
Blanvillain, Robert;Kim, Jong Heon;Ow, David W.
通讯作者：
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Hu R;Fan C;Li H;Zhang Q;Fu YF
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作者：
Haas, Brian J.;Salzberg, Steven L.;Zhu, Wei;Pertea, Mihaela;Allen, Jonathan E.;Orvis, Joshua;White, Owen;Buell, C. Robin;Wortman, Jennifer R.
通讯作者：
Wortman, Jennifer R.
影响因子：
6.9
作者：
Huseby S;Koprivova A;Lee BR;Saha S;Mithen R;Wold AB;Bengtsson GB;Kopriva S
通讯作者：
Kopriva S