Renal proteome in mice with different susceptibilities to fluorosis.
Renal proteome in mice with different susceptibilities to fluorosis.
复制标题
DOI：
10.1371/journal.pone.0053261
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Buzalaf MA
中科院分区：
文献类型：
--
作者：
Carvalho JG;Leite Ade L;Peres-Buzalaf C;Salvato F;Labate CA;Everett ET;Whitford GM;Buzalaf MA
A/J和129P3/J小鼠因其遗传背景不同，对氟斑牙的易感性不同。它们在氟(F)代谢的几个特征和水的代谢处理方面也有所不同。这项研究是为了确定氟代谢的差异是否可以通过肾脏蛋白质表达谱的差异来解释。将断奶的雄性A/J小鼠(氟斑牙易感，n = 18)和129P3/J小鼠(抗性，n = 18)配对饲养，分成三组，分别给予0、10和50ppm[F]的低氟食物和饮用水，为期7周。用2D-PAGE和LC-MS/MS分析了A/J和129P3/J株在0、10和50ppmF组的122、126和134个差异表达斑点。从中分别成功鉴定出25株、30株和32株。大多数蛋白质与代谢和细胞过程有关，其次是对刺激的反应、细胞过程的发育和调节。在F处理组，参与调节肾小管重吸收能力的蛋白PDZK-1在129P3/J小鼠的肾脏中下调。A/J和129P3/J小鼠分别表现出11种和3种独占蛋白，与F暴露无关。总之，蛋白质组学分析能够识别在F和水的代谢处理中潜在参与的蛋白质，这些蛋白质在被评估的菌株中差异表达，甚至不表达。这有助于了解氟斑牙遗传易感性的分子机制，指出关键蛋白应该在未来的研究中得到更好的解决。
A/J and 129P3/J mouse strains have different susceptibilities to dental fluorosis due to their genetic backgrounds. They also differ with respect to several features of fluoride (F) metabolism and metabolic handling of water. This study was done to determine whether differences in F metabolism could be explained by diversities in the profile of protein expression in kidneys. Weanling, male A/J mice (susceptible to dental fluorosis, n = 18) and 129P3/J mice (resistant, n = 18) were housed in pairs and assigned to three groups given low-F food and drinking water containing 0, 10 or 50 ppm [F] for 7 weeks. Renal proteome profiles were examined using 2D-PAGE and LC-MS/MS. Quantitative intensity analysis detected between A/J and 129P3/J strains 122, 126 and 134 spots differentially expressed in the groups receiving 0, 10 and 50 ppmF, respectively. From these, 25, 30 and 32, respectively, were successfully identified. Most of the proteins were related to metabolic and cellular processes, followed by response to stimuli, development and regulation of cellular processes. In F-treated groups, PDZK-1, a protein involved in the regulation of renal tubular reabsorption capacity was down-modulated in the kidney of 129P3/J mice. A/J and 129P3/J mice exhibited 11 and 3 exclusive proteins, respectively, regardless of F exposure. In conclusion, proteomic analysis was able to identify proteins potentially involved in metabolic handling of F and water that are differentially expressed or even not expressed in the strains evaluated. This can contribute to understanding the molecular mechanisms underlying genetic susceptibility to dental fluorosis, by indicating key-proteins that should be better addressed in future studies.
登录
查看更多内容
DOI：
10.2170/jjphysiol.51.371
发表时间：
2001-06-01
期刊：
JAPANESE JOURNAL OF PHYSIOLOGY
影响因子：
--
作者：
Mori, Y;Kawasaki, A;Kubota, T
通讯作者：
Kubota, T
影响因子：
1.9
作者：
Everett ET;Yin Z;Yan D;Zou F
通讯作者：
Zou F
影响因子：
7.6
作者：
Everett, ET;McHenry, MAK;Stookey, GK
通讯作者：
Stookey, GK
DOI：
10.1152/ajprenal.00182.2009
发表时间：
2010-03-01
影响因子：
4.2
作者：
Dynia, Diane W.;Steinmetz, Amy G.;Kocinsky, Hetal S.
通讯作者：
Kocinsky, Hetal S.
DOI：
10.1152/ajprenal.1995.269.5.f673
发表时间：
1995-11-01
期刊：
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY
影响因子：
--
作者：
LI, DL;APERIA, A;MEISTER, B
通讯作者：
MEISTER, B