Involvement of CD8+ T cell-mediated immune responses in LcrV DNA vaccine induced protection against lethal Yersinia pestis challenge.
Involvement of CD8+ T cell-mediated immune responses in LcrV DNA vaccine induced protection against lethal Yersinia pestis challenge.
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LcrV DNA 疫苗中 CD8 T 细胞介导的免疫反应的参与诱导了针对致命性鼠疫耶尔森氏菌攻击的保护。
DOI：
10.1016/j.vaccine.2010.12.062
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发表时间：
2011
期刊：
影响因子：
5.5
通讯作者：
Lu,Shan
中科院分区：
文献类型：
--
作者：
Wang,Shixia;Goguen,JonD;Li,Fusheng;Lu,Shan
鼠疫耶尔森菌（Yersinia pestis，Y.鼠疫（pestis）是鼠疫的病原体，鼠疫是一种高度致命的疾病，对于这种疾病，仍然没有有效的疫苗，特别是针对粘膜传播的疫苗。与许多细菌感染一样，抗原特异性抗体反应在传统上被认为是疫苗诱导的抗Y保护作用的关键（如果不是唯一负责的话）。鼠疫近年来的研究表明，T细胞免疫应答在抗Y.鼠疫杆菌感染，但有关Y.鼠疫抗原特异性T细胞免疫应答。本研究旨在鉴定鼠疫疫苗开发的主要抗原LcrV蛋白中是否存在CD 8 + T细胞表位。此外，在LcrV DNA接种的Balb/C小鼠中，CD 8 + T细胞的耗竭导致对致死性鼻内攻击的Y.鼠疫这些发现证实LcrV DNA疫苗能够引发针对该关键鼠疫抗原的特异性表位的CD 8 + T细胞免疫应答，并且CD 8 + T细胞免疫应答参与LcrV DNA疫苗引发的保护。鼠疫疫苗开发的未来研究将需要检查是否存在可检测的T细胞免疫应答，特别是CD 8 + T细胞免疫应答，将增强对Y的保护。高等动物物种或人类的鼠疫。
Yersinia pestis (Y. pestis) is the causative pathogen of plague, a highly fatal disease for which an effective vaccine, especially against mucosal transmission, is still not available. Like many bacterial infections, antigen-specific antibody responses have been traditionally considered critical, if not solely responsible, for vaccine-induced protection against Y. pestis. Studies in recent years have suggested the importance of T cell immune responses against Y. pestis infection but information is still limited about the details of Y. pestis antigen-specific T cell immune responses. In current report, studies are conducted to identify the presence of CD8+ T cell epitopes in LcrV protein, the leading antigen of plague vaccine development. Furthermore, depletion of CD8+ T cells in LcrV DNA vaccinated Balb/C mice led to reduced protection against lethal intranasal challenge of Y. pestis. These findings establish that an LcrV DNA vaccine is able to elicit CD8+ T cell immune responses against specific epitopes of this key plague antigen and that a CD8+ T cell immune response is involved in LcrV DNA vaccine-elicited protection. Future studies in plague vaccine development will need to examine if the presence of detectable T cell immune responses, in particular CD8+ T-cell immune responses, will enhance the protection against Y. pestis in higher animal species or humans.
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影响因子：
2.2
作者：
T. Taguchi;J. Mcghee;R. Coffman;K. Beagley;J. Eldridge;K. Takatsu;H. Kiyono
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抗原工程在鼠疫耶尔森氏菌 DNA 疫苗引发的保护性免疫中发挥着关键作用。
影响因子：
5.5
作者：
Wang,Shixia;Mboudjeka,Innocent;Goguen,JonD;Lu,Shan
通讯作者：
Lu,Shan
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影响因子：
5.5
作者：
E. D. Williamson;A. Stagg;S. Eley;R. Taylor;Michael Green;Steven M. Jones;R. Titball
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R. Titball
接种表达鼠疫耶尔森氏菌荚膜蛋白 F1 的质粒 DNA 可以保护小鼠免受鼠疫侵害。
影响因子：
--
作者：
H. Grosfeld;T. Bino;Y. Flashner;R. Ber;Emanuelle Mamroud;S. Lustig;B. Velan;A. Shafferman;Sara B. Cohen
通讯作者：
Sara B. Cohen
由无毒鼠疫耶尔森氏菌制备的灭活鼠疫疫苗的效力。
影响因子：
11.1
作者：
J. Williams;P. Altieri;S. Berman;J. Lowenthal;D. C. Cavanaugh
通讯作者：
D. C. Cavanaugh