Caloric restriction leads to high marrow adiposity and low bone mass in growing mice.
Caloric restriction leads to high marrow adiposity and low bone mass in growing mice.
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DOI：
10.1002/jbmr.82
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发表时间：
2010-09
影响因子：
6.2
通讯作者：
Bouxsein, Mary L.
中科院分区：
文献类型：
--
作者：
Devlin, Maureen J.;Cloutier, Alison M.;Thomas, Nishina A.;Panus, David A.;Lotinun, Sutada;Pinz, Ilka;Baron, Roland;Rosen, Clifford J.;Bouxsein, Mary L.
热量限制 (CR) 对骨骼的影响在成年啮齿类动物中得到了充分研究，包括皮质骨质量较低但骨小梁体积较高。关于 CR 如何影响年轻、快速生长的动物的骨量，人们知之甚少。这是一个重要的问题，因为人类骨骼获得过程中的低热量摄入（如神经性厌食症）与低骨量、骨折风险增加和成年后骨质疏松症有关。为了探讨这个问题，我们测试了年轻小鼠骨骼快速生长过程中热量限制对骨量和微结构的影响。在 3 周龄时，我们使雄性 C57Bl/6J 小鼠断奶，接受 30% 热量限制（CR，10% Kcal/脂肪）或正常饮食（N，10% Kcal/脂肪）。 6周龄（N=4/组）和12周龄（N=8/组）的结果包括体重、股骨长度、血清瘦素和IGF-1、全身骨矿物质密度（WBBMD，g/cm2）、股骨中段和远端的皮质和骨小梁结构、骨形成和细胞构成以及骨髓脂肪测量。与 N 相比，CR 小鼠在 6 周和 12 周龄时血清瘦素分别降低 52% 和 88%，血清 IGF-1 降低 33% 和 39%（全部 p<0.05）。 CR 小鼠体型较小，骨密度、小梁骨和皮质骨特性较低。 CR 与 N 相比，骨形成指数较低，而骨吸收指数较高（全部 p<0.01）。尽管 CR 与 N 相比，体脂百分比较低，但骨髓肥胖率显着升高（p<0.05）。年轻、生长中的小鼠的热量限制与骨骼获得受损、瘦素和 IGF-1 水平低以及骨髓肥胖有关。这些结果支持这样的假设：骨骼快速生长过程中的热量限制对皮质骨和小梁骨的质量和结构有害，而 CR 对衰老动物的潜在骨骼益处则相反。
The effects of caloric restriction (CR) on the skeleton are well studied in adult rodents, and include lower cortical bone mass but higher trabecular bone volume. Much less is known about how CR affects bone mass in young, rapidly growing animals. This is an important problem because low caloric intake during skeletal acquisition in humans, as in anorexia nervosa, is associated with low bone mass, increased fracture risk, and osteoporosis in adulthood. To explore this question, we tested the effect of caloric restriction on bone mass and microarchitecture during rapid skeletal growth in young mice. At 3 wks of age we weaned male C57Bl/6J mice onto 30% caloric restriction (CR, 10% Kcal/fat) or normal diet (N, 10% Kcal/fat). Outcomes at 6 (N=4/group) and 12 wks of age (N=8/group) included body mass, femur length, serum leptin and IGF-1, whole body bone mineral density (WBBMD, g/cm2), cortical and trabecular bone architecture at the midshaft and distal femur, bone formation and cellularity, and marrow fat measurement. Compared to N, CR mice had 52% and 88% lower serum leptin and 33% and 39% lower serum IGF-1 at 6 and 12 wks of age (p<0.05 for all). CR mice were smaller, with lower bone mineral density, trabecular and cortical bone properties. Bone formation indices were lower, while bone resorption indices were higher (p<0.01 for all) in CR vs. N. Despite having lower %body fat, bone marrow adiposity was dramatically elevated in CR vs. N (p<0.05). Caloric restriction in young, growing mice is associated with impaired skeletal acquisition, low leptin and IGF-1 levels, and high marrow adiposity. These results support the hypothesis that caloric restriction during rapid skeletal growth is deleterious to cortical and trabecular bone mass and architecture, in contrast to potential skeletal benefits of CR in aging animals.
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影响因子：
4
作者：
Bolton, JGF;Patel, S;White, S
通讯作者：
White, S
影响因子：
4
作者：
Compston, JE;McConachie, C;Jaffa, A
通讯作者：
Jaffa, A
影响因子：
6.2
作者：
Bouxsein, ML;Pierroz, DD;Ferrari, SL
通讯作者：
Ferrari, SL
DOI：
10.1001/archpediatrics.2009.18
发表时间：
2009-04-01
影响因子：
--
作者：
Anderson, Sarah E.;Whitaker, Robert C.
通讯作者：
Whitaker, Robert C.
影响因子：
5.8
作者：
Bredella, Miriam A.;Fazeli, Pouneh K.;Klibanski, Anne
通讯作者：
Klibanski, Anne