Cardiac fibrosis in mice expressing an inducible myocardial-specific Cre driver.
Cardiac fibrosis in mice expressing an inducible myocardial-specific Cre driver.
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DOI：
10.1242/dmm.010470
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发表时间：
2013-11
影响因子：
4.3
通讯作者：
Rosenthal N
中科院分区：
文献类型：
--
作者：
Lexow J;Poggioli T;Sarathchandra P;Santini MP;Rosenthal N
Tamoxifen-inducible Cre-mediated manipulation of animal genomes has achieved wide acceptance over the last decade, with numerous important studies heavily relying on this technique. Recently, a number of groups have reported transient complications of using this protocol in the heart. In the present study we observed a previously unreported focal fibrosis and depressed left-ventricular function in tamoxifen-treated αMHC-MerCreMer-positive animals in a Tβ4shRNAflox × αMHC-MerCreMer cross at 6–7 weeks following standard tamoxifen treatment, regardless of the presence of the floxed transgene. The phenotype was reproduced by treating mice from the original αMHC-MerCreMer strain with tamoxifen. In the acute phase after tamoxifen treatment, cell infiltration into the myocardium was accompanied by increased expression of pro-inflammatory cytokines (IL-1β, IL-6, TNFα, IFNγ, Ccl2) and markers of hypertrophy (ANF, BNP, Col3a1). These observations highlight the requirement for including tamoxifen-treated MerCreMer littermate controls to avert misinterpretation of conditional mutant phenotypes. A survey of the field as well as the protocols presented here suggests that controlling the parameters of tamoxifen delivery is important in avoiding the chronic MerCreMer-mediated cardiac phenotype reported here.
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影响因子：
20.1
作者：
Sohal, DS;Nghiem, M;Molkentin, JD
通讯作者：
Molkentin, JD
影响因子：
25
作者：
Madisen L;Zwingman TA;Sunkin SM;Oh SW;Zariwala HA;Gu H;Ng LL;Palmiter RD;Hawrylycz MJ;Jones AR;Lein ES;Zeng H
通讯作者：
Zeng H
影响因子：
6
作者：
Buerger, A;Rozhitskaya, O;Jay, PY
通讯作者：
Jay, PY
影响因子：
4.3
作者：
Bersell K;Choudhury S;Mollova M;Polizzotti BD;Ganapathy B;Walsh S;Wadugu B;Arab S;Kühn B
通讯作者：
Kühn B
DOI：
10.1073/pnas.0805806106
发表时间：
2009-05-05
影响因子：
11.1
作者：
Wang, Dairong;Patel, Vickas V.;FitzGerald, Garret A.
通讯作者：
FitzGerald, Garret A.