Nucleosome assembly protein 1-like 5 alleviates Alzheimer's disease-like pathological characteristics in a cell model.
Nucleosome assembly protein 1-like 5 alleviates Alzheimer's disease-like pathological characteristics in a cell model.
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DOI：
10.3389/fnmol.2022.1034766
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发表时间：
2022
影响因子：
4.8
通讯作者：
Sun, Fengxian
中科院分区：
文献类型：
--
作者：
Wang, Bingyan;Liu, Weiying;Sun, Fengxian
关键词：
阿尔茨海默病（Alzheimer's disease，AD）是神经退行性疾病中最常见的痴呆之一。核小体组装蛋白1样5（NAP 1 L5）属于NAP 1 L蛋白家族，其作为组蛋白伴侣。然而，NAP 1 L5在AD中的作用和机制尚不清楚。生物信息学分析、RT-qPCR和Western blotting结果表明，NAP 1 L5在AD患者脑组织和AD小鼠细胞模型中表达下调。NAP 1 L5过表达减轻（淀粉样蛋白-β前体蛋白）APP代谢和Tau磷酸化。我们进一步证明NAP 1 L5通过GSK 3B/Wnt/β-Catenin信号通路调节AD样病理特征。此外，我们还发现N2 a-APP 695 sw细胞中由NAP 1 L5调控的Wnt/β-Catenin信号通路是由AQP 1介导的。总之，这些结果表明，NAP 1 L5过表达具有神经保护作用，并可能作为诊断和治疗AD的潜在生物标志物和靶点。
Alzheimer’s disease (AD) remains one of the most common dementias of neurodegenerative disease-related diseases. Nucleosome assembly protein 1-like 5 (NAP1L5) belongs to the NAP1L protein family, which acts as a histone chaperone. However, the function and mechanism of NAP1L5 in AD are still unclear. Bioinformatics analysis, RT-qPCR, and Western blotting results showed that NAP1L5 was downregulated in the brain tissues of AD patients and a mouse cell model of AD. NAP1L5 overexpression alleviated (Amyloid-β precursor protein) APP metabolism and Tau phosphorylation. We further demonstrated that NAP1L5 regulated the AD-like pathological characteristics through the GSK3B/Wnt/β-Catenin signaling pathway. Moreover, we showed that the Wnt/β-Catenin signaling pathway, regulated by NAP1L5, was mediated by AQP1-mediated mechanism in N2a-APP695sw cell. In sum, these results suggested that NAP1L5 overexpression has neuroprotective effects and might act as potential biomarker and target for the diagnosis and treatment of AD.
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影响因子：
4
作者：
Brewer, Gregory J.;Herrera, Robert A.;Glabe, Charles G.
通讯作者：
Glabe, Charles G.
DOI：
10.1007/s00018-020-03464-4
发表时间：
2020-12
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Marshall KE;Vadukul DM;Staras K;Serpell LC
通讯作者：
Serpell LC
影响因子：
3.7
作者：
Monfared, Amir Abbas Tahami;Byrnes, Michael J.;White, Leigh Ann;Zhang, Quanwu
通讯作者：
Zhang, Quanwu
影响因子：
6.1
作者：
Cervellati C;Valacchi G;Zuliani G
通讯作者：
Zuliani G
影响因子：
11.4
作者：
Cheignon C;Tomas M;Bonnefont-Rousselot D;Faller P;Hureau C;Collin F
通讯作者：
Collin F