HOXA-AS2 promotes type I endometrial carcinoma via miRNA-302c-3p-mediated regulation of ZFX
HOXA-AS2 promotes type I endometrial carcinoma via miRNA-302c-3p-mediated regulation of ZFX
复制标题
HOXA-AS2 通过 miRNA-302c-3p 介导的 ZFX 调节促进 I 型子宫内膜癌
DOI：
10.1186/s12935-020-01443-0
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发表时间：
2020-07
影响因子：
5.8
通讯作者：
Ma Xiaoxin
中科院分区：
文献类型：
--
作者：
Song Ning（）;Zhang Ying;Kong Fanfei;Yang Hui;Ma Xiaoxin
背景HOXA簇反义RNA2(HOXA-AS2)是一种长链非编码RNA，在多种恶性肿瘤的行为中起重要作用。方法采用定量逆转录聚合酶链式反应(QRT-PCR)和免疫印迹法检测Hoxa-AS2、miRNA-302C-3p、转录因子锌指X染色体蛋白(ZFX)和几丁质酶样蛋白YKL-40在子宫内膜癌中的表达。通过荧光素酶报告和qRT-PCR实验确定HOXA-AS2与miRNA-302C-3p的潜在结合位点。结果子宫内膜癌组织中HOXA-AS2表达水平显著高于正常子宫内膜组织。上调的HOXA-AS2促进I型子宫内膜癌细胞的侵袭和增殖。HOXA-AS2通过与miRNA-302C-3p结合来沉默miRNA-302C-3p。MiRNA-302C-3p负性调控ZFX和YKL-40。因此，HOXA-AS2通过miRNA-302C-3P介导的ZFX调控促进了I型子宫内膜癌的发生发展。结论HOXA-AS2可作为治疗I型子宫内膜癌的新靶点。
BackgroundHOXA cluster antisense RNA2 (HOXA-AS2), a long-chain non-coding RNA, plays an important role in the behavior of various malignant tumors. The roles of HOXA-AS2 in endometrial cancer remain unclear.MethodsWe test expression levels of HOXA-AS2, miRNA-302c-3p, the transcription factor zinc finger X-chromosomal protein (ZFX), and the chitinase-like protein YKL-40 in endometrial carcinoma by qRT-PCR and western blotting. Luciferase reporter and qRT-PCR assays were conducted to identify potential binding sites of HOXA-AS2 to miRNA-302c-3p. Cell cycle, migration and invasion ability of endometrial cancer cells were investigated using flow-cytometric analysis, CCK-8 and transwell assays, respectively.ResultsHOXA-AS2 levels were significantly increased in endometrial cancer specimens compared to normal endometrial specimens. Upregulated HOXA-AS2 promoted invasion and proliferation of type I endometrial cancer cells. HOXA-AS2 silenced miRNA-302c-3p by binding to it. MiRNA-302c-3p negatively regulates ZFX and YKL-40. Thus HOXA-AS2 promotes the development of type I endometrial cancer via miRNA-302c-3p-mediated regulation of ZFX.ConclusionsThese findings suggest that HOXA-AS2 can act as a new therapeutic target for type I endometrial cancer.
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影响因子：
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作者：
Low D;Subramaniam R;Lin L;Aomatsu T;Mizoguchi A;Ng A;DeGruttola AK;Lee CG;Elias JA;Andoh A;Mino-Kenudson M;Mizoguchi E
通讯作者：
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影响因子：
5.3
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影响因子：
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