Isolation of two novel genes, down-regulated in gastric cancer.
Isolation of two novel genes, down-regulated in gastric cancer.
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DOI：
10.1111/j.1349-7006.2000.tb00967.x
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发表时间：
2000-05
期刊：
影响因子：
--
通讯作者：
Kato I
中科院分区：
文献类型：
--
作者：
Yoshikawa Y;Mukai H;Hino F;Asada K;Kato I
利用差异显示技术，我们发现了与正常胃粘膜相比，人类胃癌组织中下调的两个基因。northern blotting分析和RT - PCR证实了这些基因在胃癌组织中的下调表达。一种是CA11，这是一种主要在胃中表达的新基因，在所有被检测的胃癌细胞系中都被耗尽。另一个基因GC36与消化道特异性钙蛋白酶基因nCL‐4同源。在分化型和低分化型胃癌细胞系中，GC36和nCL‐4的表达均被抑制或缺失。这是首次报道在胃癌中表达下调且频率相当高的基因。
Using a differential display technique, we identified two genes that are down‐regulated in human gastric cancer tissue as compared to normal gastric mucosa. The down‐regulated expression of these genes in gastric cancer tissue was confirmed by northern blotting analysis and RT‐PCR. One, CA11, was a novel gene expressed predominantly in the stomach and was depleted in all of the gastric cancer cell lines examined. The other gene, GC36, was homologous to the digestive tract‐specific calpain gene, nCL‐4. The expression of both GC36 and nCL‐4 was suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types. This is the first report of genes, the expression of which is down‐regulated with considerable frequency in gastric cancer.
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